Pumping of mammalian cells with a nozzle-diffuser micropumpReport as inadecuate




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Published in: Lab On a Chip (ISSN: 1473-0197), vol. 5, num. 10, p. 1083-1088 Royal Society of Chemistry, 2005

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.

Keywords: Jurkat cells ; Hybridoma cells ; Lab-On-a-Chip ; Micropump ; Valve-less ; Nozzle ; Diffuser Reference LMIS2-ARTICLE-2005-007doi:10.1039/b504468eView record in Web of Science





Author: Yamahata, C.; Vandevyver, C.; Lacharme, F.; Izewska, P.; Vogel, H.; Freitag, R.; Gijs, M. A. M.

Source: https://infoscience.epfl.ch/record/55891?ln=en



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