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Abstract: Two-dimensional gel electrophoresis has been instrumental in the birth anddevelopments of proteomics, although it is no longer the exclusive separationtool used in the field of proteomics. In this review, a historical perspectiveis made, starting from the days where two-dimensional gels were used and theword proteomics did not even exist. The events that have led to the birth ofproteomics are also recalled, ending with a description of the now well-knownlimitations of two-dimensional gels in proteomics. However, theoften-underestimated advantages of two-dimensional gels are also underlined,leading to a description of how and when to use two-dimensional gels for thebest in a proteomics approach. Taking support of these advantages robustness,resolution, and ability to separate entire, intact proteins, possible futureapplications of this technique in proteomics are also mentioned.



Author: Thierry Rabilloud BBSI, Mireille Chevallet BBSI, Sylvie Luche BBSI, C├ęcile Lelong BBSI

Source: https://arxiv.org/







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