Norovirus translation requires an interaction between the C terminus of VPg and eIF4GReport as inadecuate


Norovirus translation requires an interaction between the C terminus of VPg and eIF4G


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Publication Date: 2014-06-13

Journal Title: Journal of Biological Chemistry

Publisher: The American Society for Biochemistry and Molecular Biology

Language: English

Type: Article

Metadata: Show full item record

Citation: Chung, L., Bailey, D., Leen, E. N., Emmott, E. P., Chaudhry, Y., Roberts, L. O., Curry, S., et al. (2014). Norovirus translation requires an interaction between the C terminus of VPg and eIF4G. Journal of Biological Chemistry

Description: This is the final published version. It's also available on the publisher's website at: http://www.jbc.org/content/early/2014/06/13/jbc.M114.550657.abstract

Abstract: Viruses have evolved a variety of mechanisms to usurp the host cell translation machinery to enable translation of the viral genome in the presence of high levels of cellular mRNAs. Noroviruses, a major cause of gastroenteritis in man, have evolved a mechanism that relies on the interaction of translation initiation factors with the virus encoded VPg protein covalently linked to the 5′ end of the viral RNA. To further characterise this novel mechanism of translation initiation, we have used proteomics to identify the components of the norovirus translation initiation factor complex. This approach revealed that VPg binds directly to the eIF4F complex, with a high affinity interaction occurring between VPg and eIF4G. Mutational analyses indicated that the C-terminal region of VPg is important for the VPg-eIF4G interaction; viruses with mutations that alter or disrupt this interaction are debilitated or non-viable. Our results shed new light on the unusual mechanisms of protein-directed translation initiation.

Keywords: Translation, Translation initiation factors, Virus, eIF4E, eIF4G, Norovirus, VPg

Sponsorship: This work was supported by funding from the BBSRC (BB/I012303/1) and the Wellcome Trust (WT097997MA) to IG, funding from BBSRC to LR and NL (BB/I01232X/1), as well as to SC (BB/J001708/1). IG is a Wellcome Senior Fellow.

Embargo Lift Date: 2030-07-08

Identifiers:

This record's URL: http://dx.doi.org/10.1074/jbc.M114.550657http://www.repository.cam.ac.uk/handle/1810/245433

Rights: Creative Commons Attribution License 2.0 UK, Attribution 2.0 UK: England & Wales

Licence URL: http://creativecommons.org/licenses/by/2.0/uk/





Author: Chung, LilianeBailey, DalanLeen, Eoin N.Emmott, Edward P.Chaudhry, YasminRoberts, Lisa O.Curry, StephenLocker, NicolasGoodfellow,

Source: https://www.repository.cam.ac.uk/handle/1810/245433



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