A microfluidic platform for trapping, releasing and super-resolution imaging of single cellsReport as inadecuate


A microfluidic platform for trapping, releasing and super-resolution imaging of single cells


A microfluidic platform for trapping, releasing and super-resolution imaging of single cells - Download this document for free, or read online. Document in PDF available to download.

Publication Date: 2016-03-25

Journal Title: Sensors and Actuators B: Chemical

Publisher: Elsevier

Volume: 232

Pages: 680-691

Language: English

Type: Article

This Version: VoR

Metadata: Show full item record

Citation: Zhou, Y., Basu, S., Wohlfahrt, K. J., Lee, S. F., Klenerman, D., Laue, E. D., & Seshia, A. A. (2016). A microfluidic platform for trapping, releasing and super-resolution imaging of single cells. Sensors and Actuators B: Chemical, 232 680-691. https://doi.org/10.1016/j.snb.2016.03.131

Abstract: A multi-layer device, combining hydrodynamic trapping with microfluidic valving techniques, has been developed for on-chip trapping, releasing and manipulation of single cells and particles. Such a device contains a flow layer with trapping channels to capture single particles or cells and a control layer with valve channels to selectively control the trap and release processes. Particles and cells have been successfully trapped and released using the proposed device. The device enables the trapping of single particles with a trapping efficiency of greater than 95%, and has the advantage that single particles and cells can be trapped, released and manipulated by simply controlling corresponding valves. Moreover, the trap and release processes are found to be compatible with biological samples like cells. Our device allows immobilising of large numbers of single cells in a few minutes, significantly easing the experiment setup for single-cell characterisation and offering a stable platform for both single-molecule and super-resolution imaging. Proof-of-concept super-resolution imaging experiments with mouse embryonic stem cells (mESCs) have been conducted by exploiting super-resolution photoactivated localisation microscopy (PALM). Cells and nuclei were stably trapped and imaged. Centromeres of ∼200 nm size could be identified with a localisation precision of <15 nm.

Keywords: hydrodynamic trapping, particle manipulation, single cell analysis, super-resolution imaging, embryonic stem cells

Relationships: Is supplemented by: https://doi.org/10.17863/CAM.589

Sponsorship: Funding from the Biotechnology and Biological Sciences Research Council (Grant BB/K013726/1) is gratefully acknowledged. The authors also thank the Royal Society for the University Research Fellowship of Dr. Steven F. Lee (UF120277).

Identifiers:

External DOI: https://doi.org/10.1016/j.snb.2016.03.131

This record's URL: https://www.repository.cam.ac.uk/handle/1810/254897



Rights: Attribution 4.0 International

Licence URL: https://creativecommons.org/licenses/by/4.0/





Author: Zhou, YingBasu, SrinjanWohlfahrt, Kai J.Lee, Steven F.Klenerman, DavidLaue, Ernest D.Seshia, Ashwin A.

Source: https://www.repository.cam.ac.uk/handle/1810/254897



DOWNLOAD PDF




Related documents