Global reorganisation of $ extit{cis}$-regulatory units upon lineage commitment of human embryonic stem cellsReport as inadecuate


Global reorganisation of $	extit{cis}$-regulatory units upon lineage commitment of human embryonic stem cells


Global reorganisation of $ extit{cis}$-regulatory units upon lineage commitment of human embryonic stem cells - Download this document for free, or read online. Document in PDF available to download.

Publication Date: 2017-03-23

Journal Title: eLife

Publisher: eLife Sciences Publications Ltd

Volume: 6

Number: e21926

Language: English

Type: Article

This Version: VoR

Metadata: Show full item record

Citation: Freire-Pritchett, P., Schoenfelder, S., Várnai, C., Wingett, S., Cairns, J., Collier, A., García-Vílchez, R., et al. (2017). Global reorganisation of $\textit{cis}$-regulatory units upon lineage commitment of human embryonic stem cells. eLife, 6 (e21926)https://doi.org/10.7554/eLife.21926

Abstract: Long-range $\textit{cis}$-regulatory elements such as enhancers coordinate cell-specific transcriptional programmes by engaging in DNA looping interactions with target promoters. Deciphering the interplay between the promoter connectivity and activity of $\textit{cis}$-regulatory elements during lineage commitment is crucial for understanding developmental transcriptional control. Here, we use Promoter Capture Hi-C to generate a high-resolution atlas of chromosomal interactions involving ~22,000 gene promoters in human pluripotent and lineage-committed cells, identifying putative target genes for known and predicted enhancer elements. We reveal extensive dynamics of $\textit{cis}$-regulatory contacts upon lineage commitment, including the acquisition and loss of promoter interactions. This spatial rewiring occurs preferentially with predicted changes in the activity of $\textit{cis}$-regulatory elements and is associated with changes in target gene expression. Our results provide a global and integrated view of promoter interactome dynamics during lineage commitment of human pluripotent cells.

Keywords: chromosomes, cis-regulatory units, developmental biology, gene regulation, genes, human, human embryonic stem cells, lineage committment, nuclear architecture, promoter capture Hi-C, stem cells

Sponsorship: Wellcome Trust (WT093736), Biotechnology and Biological Sciences Research Council (BB/J004480/1), Medical Research Council (MR/J003808/1)

Embargo Lift Date: 2100-01-01

Identifiers:

External DOI: https://doi.org/10.7554/eLife.21926

This record's URL: https://www.repository.cam.ac.uk/handle/1810/264775



Rights: Attribution 4.0 International

Licence URL: http://creativecommons.org/licenses/by/4.0/





Author: Freire-Pritchett, PSchoenfelder, SVárnai, CWingett, SWCairns, JCollier, AJGarcía-Vílchez, RFurlan-Magaril, MOsborne, CSFraser,

Source: https://www.repository.cam.ac.uk/handle/1810/264775



DOWNLOAD PDF




Related documents