Essential genes for in vitro growth of the endophyte Herbaspirillum seropedicae SmR1 revealed by transposon insertion sequencingReport as inadecuate


Essential genes for in vitro growth of the endophyte Herbaspirillum seropedicae SmR1 revealed by transposon insertion sequencing


Essential genes for in vitro growth of the endophyte Herbaspirillum seropedicae SmR1 revealed by transposon insertion sequencing - Download this document for free, or read online. Document in PDF available to download.

Publication Date: 2016-09-02

Journal Title: Applied and Environmental Microbiology

Publisher: American Society for Microbiology

Language: English

Type: Article

This Version: AM

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Citation: Rosconi, F., de Vries, S. P. W., Baig, A., Fabiano, E., & Grant, A. J. (2016). Essential genes for in vitro growth of the endophyte Herbaspirillum seropedicae SmR1 revealed by transposon insertion sequencing. Applied and Environmental Microbiology https://doi.org/10.1128/AEM.02281-16

Description: This is the author accepted manuscript. The final version is available from the American Society for Microbiology at http://dx.doi.org/10.1128/AEM.02281-16.

Abstract: The interior of plants contains microorganisms (referred to as endophytes) distinct to those present in the root surface or in the surrounding soil. Herbaspirillum seropedicae strain SmR1, belonging to the β- Proteobacterium, is an endophyte that colonizes crops, including rice, maize, sugarcane and sorghum. Different approaches have revealed genes and pathways regulated during the interaction of H. seropedicae with its plant hosts. However, functional genomic analysis of transposon (Tn) mutants has been hampered by the lack of genetic tools. Here we successfully employed a combination of in vivo high-density mariner Tn mutagenesis and targeted sequencing of Tn insertion sites (Tn-seq) in H. seropedicae SmR1. The analysis of multiple gene-saturating Tn libraries revealed that 395 genes are essential for growth of H. seropedicae SmR1 in TY medium. A comparative analysis with the “database of essential genes” known as DEG, showed that 25 genes are uniquely essential in H. seropedicae SmR1. The developed Tn mutagenesis protocol and the generated gene-saturating Tn libraries will assist with the elucidation of the genetic mechanisms of H. seropedicae endophytic lifestyle.

Keywords: Herbaspirillum, endophytes, Tn-seq

Sponsorship: This work was funded by an EMBO ASTF 425-2014 short-term fellowship awarded to FR and conducted in the laboratory of AJG. SPWdV and AB were funded by BBSRC grant BB/K004514/1.

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External DOI: https://doi.org/10.1128/AEM.02281-16

This record's URL: https://www.repository.cam.ac.uk/handle/1810/260916



Rights: Attribution 4.0 International

Licence URL: http://creativecommons.org/licenses/by/4.0/





Author: Rosconi, Federicode Vries, Stefan P. W.Baig, AbiyadFabiano, ElenaGrant, Andrew J.

Source: https://www.repository.cam.ac.uk/handle/1810/260916



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