Analysis of the expression patterns, Subcellular localisations and interaction partners of drosophila proteins using a pigp protein trap libraryReport as inadecuate


Analysis of the expression patterns, Subcellular localisations and interaction partners of drosophila proteins using a pigp protein trap library


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Publication Date: 2014-10-07

Journal Title: Development

Publisher: The Company of Biologists

Volume: 141

Issue: 20

Pages: 3994-4005

Language: English

Type: Article

Metadata: Show full item record

Citation: Lowe, N., Rees, J. S., Roote, J., Ryder, E., Armean, I. M., Johnson, G., Drummond, E., et al. (2014). Analysis of the expression patterns, Subcellular localisations and interaction partners of drosophila proteins using a pigp protein trap library. Development, 141 (20), 3994-4005.

Description: This is the final version of the article. It was first available from The Company of Biologists via http://dx.doi.org/10.1242/dev.111054

Abstract: Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying an artificial exon encoding yellow fluorescent protein (YFP) and protein affinity tags. From screening 41 million embryos, we recovered 616 verified independent YFP-positive lines representing protein traps in 374 genes, two-thirds of which had not been tagged in previousPelement protein trap screens. Over 20 different research groupsthen characterized the expression patterns of the tagged proteins in a variety of tissues and at several developmental stages. In parallel, we purified many of the tagged proteins from embryos using the affinity tags and identified co-purifying proteins by mass spectrometry. The fly stocks are publicly available through the Kyoto Drosophila Genetics Resource Center. All our data are available via an open access database (Flannotator), which provides comprehensive information on the expression patterns, subcellular localisations and in vivo interaction partners of the trapped proteins. Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures.

Keywords: Affinity purification, Cytoophidia, Live imaging, piggyBac, Protein trap

Sponsorship: This work was supported by a project grant from the Wellcome Trust [076739], by a Wellcome Trust Principal Research Fellowship to D.StJ. [049818 and 080007], and by core support from the Wellcome Trust [092096] and Cancer Research UK [A14492].

Identifiers:

This record's URL: http://dx.doi.org/10.1242/dev.111054https://www.repository.cam.ac.uk/handle/1810/253317

Rights: Attribution 2.0 UK: England & Wales

Licence URL: http://creativecommons.org/licenses/by/2.0/uk/





Author: Lowe, NickRees, Johanna S.Roote, JohnRyder, EdArmean, Irina M.Johnson, GlynnisDrummond, EmmaSpriggs, HelenDrummond, JennyMagbanua,

Source: https://www.repository.cam.ac.uk/handle/1810/253317



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