Vol 9: Induction of Neutralizing Antibody Response against Four Dengue Viruses in Mice by Intramuscular Electroporation of Tetravalent DNA Vaccines.Report as inadecuate



 Vol 9: Induction of Neutralizing Antibody Response against Four Dengue Viruses in Mice by Intramuscular Electroporation of Tetravalent DNA Vaccines.


Vol 9: Induction of Neutralizing Antibody Response against Four Dengue Viruses in Mice by Intramuscular Electroporation of Tetravalent DNA Vaccines. - Download this document for free, or read online. Document in PDF available to download.

Download or read this book online for free in PDF: Vol 9: Induction of Neutralizing Antibody Response against Four Dengue Viruses in Mice by Intramuscular Electroporation of Tetravalent DNA Vaccines.
This article is from PLoS ONE, volume 9.AbstractDNA vaccine against dengue is an interesting strategy for a prime-boost approach. This study evaluated neutralizing antibody NAb induction of a dengue tetravalent DNA TDNA vaccine candidate administered by intramuscular-electroporation IM-EP and the benefit of homologous TDNA boosting in mice. Consensus humanized pre-membrane prM and envelope E of each serotypes, based on isolates from year 1962–2003, were separately cloned into a pCMVkan expression vector. ICR mice, five-six per group were immunized for three times 2-week interval with TDNA at 100 µg group I; 25 µg-monovalent or 10 µg group II; 2.5 µg-monovalent. In group I, mice received an addtional TDNA boosting 13 weeks later. Plaque reduction neutralization tests PRNT were performed at 4 weeks post-last immunization. Both 100 µg and 10 µg doses of TDNA induced high NAb levels against all DENV serotypes. The median PRNT50 titers were comparable among four serotypes of DENV after TDNA immunization. Median PRNT50 titers ranged 240–320 in 100 µg and 160–240 in 10 µg groups p = ns. A time course study of the 100 µg dose of TDNA showed detectable NAb at 2 weeks after the second injection. The NAb peaked at 4 weeks after the third injection then declined over time but remained detectable up to 13 weeks. An additional homologous TDNA boosting significantly enhanced the level of NAb from the nadir for at least ten-fold p



Author: Prompetchara, Eakachai; Ketloy, Chutitorn; Keelapang, Poonsook; Sittisombut, Nopporn; Ruxrungtham, Kiat

Source: https://archive.org/







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