Isolation and prepurification of active compounds in venom from pelagia noctiluca (scyphozoa: pelagiidae) from the caribbean sea Report as inadecuate




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NL Lucio-Martínez ;Ciencias Marinas 2011, 37 (3)

Author: J Sánchez-Rodríguez

Source: http://www.redalyc.org/


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Ciencias Marinas ISSN: 0185-3880 cmarinas@uabc.mx Universidad Autónoma de Baja California México Sánchez-Rodríguez, J; Lucio-Martínez, NL Isolation and prepurification of active compounds in venom from Pelagia noctiluca (Scyphozoa: Pelagiidae) from the Caribbean Sea Ciencias Marinas, vol.
37, núm.
3, septiembre, 2011, pp.
369-377 Universidad Autónoma de Baja California Ensenada, México Available in: http:--www.redalyc.org-articulo.oa?id=48020755010 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Ciencias Marinas (2011), 37(3): 369–377 Isolation and prepurification of active compounds in venom from Pelagia noctiluca (Scyphozoa: Pelagiidae) from the Caribbean Sea C M Aislamiento y prepurificación de los compuestos activos presentes en el veneno de Pelagia noctiluca (Scyphozoa: Pelagiidae) del Mar Caribe J Sánchez-Rodríguez*, NL Lucio-Martínez Unidad Académica de Sistemas Arrecifales, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Prolongación Niños Héroes s-n, Domicilio conocido, Puerto Morelos CP 77580, Quintana Roo, México. * Corresponding author.
E-mail: judithsa@cmarl.unam.mx ABSTRACT.
Crude extract from Pelagia noctiluca was purified using liquid chromatography.
The isolated fractions were tested in sea crabs (Ocypode quadrata) and neurotoxic activity was observed.
The protein content of the crude extract, quantified by the Bradford method, was 0.25 mg mL–1.
Polyacrylamide gel electrophoresis was used to determine the apparent molecular weight of the different fractions of the crude extract, and several bands were identified: 34, 48, and between 90 and 200 kDa.
Haemolytic activity in human blood type A was 100% with 1.1 g mL–1 and 50% with 0.98 g mL–1...





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