Substrate Micropatterning as a New in Vitro Cell Culture System to Study MyelinationReport as inadecuate




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Myelination is a highly regulated developmentalprocess whereby oligodendrocytes in the central nervous systemand Schwann cells in the peripheral nervous system ensheathe axonswith a multilayered concentric membrane. Axonal myelinationincreases the velocity of nerve impulse propagation. In this work,we present a novel in vitro system for coculturing primary dorsal rootganglia neurons along with myelinating cells on a highly restrictiveand micropatterned substrate. In this new coculture system, neuronssurvive for several weeks, extending long axons on defined Matrigeltracks. On these axons, myelinating cells can achieve robust myelination, as demonstrated by the distribution of compact myelinand nodal markers. Under these conditions, neurites and associated myelinating cells are easily accessible for studies on themechanisms of myelin formation and on the effects of axonal damage on the myelin sheath.Nota general

Artículo de publicación ISI



Author: Liazoghli, Dalinda; - Roth, Alejandro D.; - Thostrup, Peter; - Colman, David R.; -

Source: http://repositorio.uchile.cl/



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