Frequency distribution of genes encoding aminoglycoside modifying enzymes in uropathogenic E. coli isolated from Iranian hospitalReport as inadecuate




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BMC Research Notes

, 7:842

Microbiology

Abstract

BackgroundEscherichia coli is considered as the most common cause of urinary tract infection UTI and acquired multiple resistances to a wide range of antibiotics such as aminoglycosides. Enzymatic alteration of aminoglycosides AMEs by aminoglycoside- modifying enzymes is the main mechanism of resistance to these antibiotics in E. coli. The aim of this study was detection and investigation of frequency of genes encoding aminoglycoside modifying enzymes aac3-IIa and ant2′′-Ia in UPEC isolated from hospitalized patients in teaching hospital of Tehran, Iran.

FindingsA total of 276 UPEC were obtained from Urine samples in a hospital from Tehran. Antibiotic susceptibility to aminoglycosides was determined by disk diffusion method according CLSI guidelines in UPEC isolates. MICs of target antibiotics were determined by agar dilution method. All isolates were screened for the presence of the AMEs genes using the PCR. The results of disk diffusion showed 21%, 24.6%, 23.18%, 3.62% and 6.15% of isolates were resistant to Gentamicin, Tobramycin, Kanamicin, Amikacin and Netilmicin respectively. The agar dilution’s results MICs were high, 66.19% for Gentamicin. The aac 3-IIa and ant2″-Ia genes were detected in 78.87% and 47.88% of isolates respectively.

ConclusionsThis study shows the high frequency of genes encoding AMEs aac3-IIa and ant2-Ia genes and their relationship between different aminoglycoside resistance phenotypes.

KeywordsUPEC Aminoglycoside modifying enzymes and UTI Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-842 contains supplementary material, which is available to authorized users.

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Author: Neda Soleimani - Mahdi Aganj - Liaqat Ali - Leili Shokoohizadeh - Türkân Sakinc

Source: https://link.springer.com/







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