MicroRNA in exosomes isolated directly from the liver circulation in patients with metastatic uveal melanomaReport as inadecuate




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BMC Cancer

, 14:962

Genetics, genomics and epigenetics

Abstract

BackgroundUveal melanoma is a tumour arising from melanocytes of the eye, and 30 per cent of these patients develop liver metastases. Exosomes are small RNA containing nano-vesicles released by most cells, including malignant melanoma cells. This clinical translational study included patients undergoing isolated hepatic perfusion IHP for metastatic uveal melanoma, from whom exosomes were isolated directly from liver perfusates. The objective was to determine whether exosomes are present in the liver circulation, and to ascertain whether these may originate from melanoma cells.

MethodsExosomes were isolated from the liver perfusate of twelve patients with liver metastases from uveal melanoma undergoing IHP. Exosomes were visualised by electron microscopy, and characterised by flow cytometry, Western blot and real-time PCR. Furthermore, the concentration of peripheral blood exosomes were measured and compared to healthy controls.

ResultsThe liver perfusate contained Melan-A positive and RNA containing exosomes, with similar miRNA profiles among patients, but dissimilar miRNA compared to exosomes isolated from tumor cell cultures. Patients with metastatic uveal melanoma had a higher concentration of exosomes in their peripheral venous blood compared to healthy controls.

ConclusionsMelanoma exosomes are released into the liver circulation in metastatic uveal melanoma, and is associated with higher concentrations of exosomes in the systemic circulation. The exosomes isolated directly from liver circulation contain miRNA clusters that are different from exosomes from other cellular sources.

KeywordsExosomes Extracellular vesicles Biomarker microRNA Uveal melanoma Liver perfusion Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-14-962 contains supplementary material, which is available to authorized users.

Maria Eldh, Roger Olofsson Bagge contributed equally to this work.

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Author: Maria Eldh - Roger Olofsson Bagge - Cecilia Lässer - Joar Svanvik - Margareta Sjöstrand - Jan Mattsson - Per Lindnér - D

Source: https://link.springer.com/







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