Higher frequency of regulatory T cells in granulocyte colony-stimulating factor G-CSF-primed bone marrow grafts compared with G-CSF-primed peripheral blood graftsReport as inadecuate




Higher frequency of regulatory T cells in granulocyte colony-stimulating factor G-CSF-primed bone marrow grafts compared with G-CSF-primed peripheral blood grafts - Download this document for free, or read online. Document in PDF available to download.

Journal of Translational Medicine

, 13:145

First Online: 07 May 2015Received: 28 July 2014Accepted: 27 April 2015DOI: 10.1186-s12967-015-0507-z

Cite this article as: Zhao, XY., Wang, YT., Mo, XD. et al. J Transl Med 2015 13: 145. doi:10.1186-s12967-015-0507-z

Abstract

BackgroundRegulatory T cells Treg in allografts are important for the prevention of graft-versus-host disease GVHD post-transplantation. The aim of this study was to compare the contents of Tregs and effector T cells in granulocyte colony-stimulating factor G-CSF-primed bone marrow grafts G-BM and peripheral blood grafts G-PB.

MethodG-BM and G-PB were obtained from 20 allogeneic donors. T-cell subgroups, including conventional T cells and different types of Treg cells, as well as the percentage of Ki67 expression on CD4CD25Foxp3 Treg cells, were analyzed using flow cytometry. The levels of interferon-γ IFN-γ and interleukin-17 IL-17 secreted by T cells stimulated with PMA and ionomycin were also determined by flow cytometry.

ResultsThe percentage of CD4CD25CD127CD62L Treg cells was significantly higher in the G-BM group, with higher proportions of CD45RA naïve Treg cells and higher expression of CD69 on Treg cells in G-BM P < 0.05. The percentage of Ki67 expression in CD4CD25Foxp3 Treg cells in G-BM was significantly higher than that on G-PB. The suppressive functions of Treg cells in inhibiting T-cell activation were comparable between G-BM and G-PB. The proportions of CD4CD25CD69 Treg subsets as well as Th1 cells in G-BM were also significantly higher than those in G-PB P < 0.001. The proportions of conventional T cells and Th17 effector cells were comparable in G-BM compared with those in G-PB. Thus, the ratio of conventional T cells and CD4CD25CD127 regulatory T cells were lower in G-BM than that in G-PB P = 0.014.

ConclusionIn addition to the much higher T-cell counts in G-PB grafts that may contribute to more severe GVHD, the higher frequency of Treg cells and lower ratio of conventional T cells to Treg cells in G-BM compared with G-PB grafts might reduce GVHD post-transplantation in G-BM compared with G-PB transplantation.

KeywordsRegulatory T cells Effector T cells G-BM G-PB Xiang-Yu Zhao and Yu-Tong Wang contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-s12967-015-0507-z contains supplementary material, which is available to authorized users.

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Author: Xiang-Yu Zhao - Yu-Tong Wang - Xiao-Dong Mo - Xiao-Su Zhao - Ya-Zhe Wang - Ying-Jun Chang - Xiao-Jun Huang

Source: https://link.springer.com/







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