Prognostic relevance of LGALS3BP in human colorectal carcinomaReport as inadecuate




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Journal of Translational Medicine

, 13:248

First Online: 30 July 2015Received: 10 May 2015Accepted: 16 July 2015DOI: 10.1186-s12967-015-0606-x

Cite this article as: Piccolo, E., Tinari, N., D’Addario, D. et al. J Transl Med 2015 13: 248. doi:10.1186-s12967-015-0606-x

Abstract

BackgroundA previous report has shown that LGALS3BP also known as 90K or Mac-2 BP has antitumor activity in colorectal cancer CRC via suppression of Wnt signalling with a novel mechanism of ISGylation-dependent ubiquitination of β-catenin. The role of LGALS3BP in CRC prognosis was investigated.

MethodsThe role of LGALS3BP on CRC progression and clinical prognosis was analyzed by combining cell cultures, in vitro assays, and immunohistochemistry.

ResultsSilencing of LGALS3BP in HCT-116 human colon cancer cells resulted in enhanced β-catenin expression that was reversed by addition of human recombinant LGALS3BP. Moreover, intra-tumor delivery of LGALS3BP reduced tumor growth of xenografts originating from LGALS3BP-silenced HCT-116 cells. Finally, in a series of 196 CRC patients, LGALS3BP expression in tumor tissue associated with clinical outcome. Patients with high LGALS3BP expression had lower risk of relapse and a longer overall survival time than those with low LGALS3BP expression. Multivariate analyses confirmed LGALS3BP expression status as the only independent prognostic factor of survival.

ConclusionsThese results provide evidence that low expression of LGALS3BP participates in malignant progression of CRC and implicates poor prognosis, highlighting its augmentation as a potential therapeutic approach.

KeywordsLGALS3BP β-Catenin Colorectal cancer Prognosis AbbreviationsLGALS3BPlectin, galactoside-binding, soluble, 3 binding protein

CRCcolorectal cancer

RPMI-1640Roswell Park Memorial Institute

FBSfoetal bovine serum

ELISAenzyme-linked immunosorbent assay

EDTAethylenediaminetetraacetic acid

RIPAradioimmunoprecipitation assay

PBSphosphate buffered saline

HRPhorse radish peroxidase

TMAtissue microarray

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Author: Enza Piccolo - Nicola Tinari - Domenica D’Addario - Cosmo Rossi - Valentina Iacobelli - Rossana La Sorda - Rossano Lattan

Source: https://link.springer.com/







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