Nutritional evaluation, antioxidant studies and quantification of poly phenolics, in Roscoea purpurea tubersReport as inadecuate




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BMC Research Notes

, 8:324

Complementary and AlternativeMedicine

Abstract

BackgroundRoscoea purpurea Zingiberaceae is commonly known as -kakoli-. Traditionally, various parts like leaves, roots and flower etc. are used for the treatment of diabetic, hypertension, diarrhea, fever, inflammation etc. In Nepal tubers are boiled for edible purpose and also used in traditional veterinary medicine. The study aims for nutritional characterization, chemical profiling of R. purpurea tubers methanol extract RPE along with evaluation of its anti-oxidant activity. Physicochemical and nutritional content were estimated as per standard protocols. Chemical profiling of markers includes method optimization, identification and quantification of bioactive poly phenolics through HPTLC. Anti oxidant potential RPE was analyzed via. Total phenolics TPC, total flavonoids TFC, reducing power assay, DPPH and β-carotene bleaching model.

ResultsPhysicochemical and nutritional standards were established. Kaempferol 0.30%, vanillic acid 0.27%, protocatechuic 0.14%, syringic 0.80% and ferulic acid 0.05% were identified and then quantified. TPC and TFC content were found to be 7.10 ± 0.115 and 6.10 ± 0.055%, reducing power of extract also increases linearly r = 0.946 with concentration, similar to standards. IC50 value of extract in DPPH and β-carotene bleaching model was observed at 810.66 ± 1.154 and 600.66 ± 1.154 µg-ml, which is significantly different from standards p < 0.05. Although there is a positive, significant correlation between the phenolic and flavonoid content with anti oxidant activity of extract.

ConclusionThus, study will authenticates the identity, utility of herb as nutrient supplement and an important medicinal plant having promising pharmacological activities for further elaborated-extended investigation work.

KeywordsRoscoea purpurea Nutritional characterization HPTLC Invitro assays Poly phenolics  Download fulltext PDF



Author: Ankita Misra - Sharad Srivastava - Shikhar Verma - Ajay Kumar Singh Rawat

Source: https://link.springer.com/







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