Therapeutic Potential of Polar and Non-Polar Extracts of Cyanthillium cinereum In VitroReport as inadecuate




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Evidence-Based Complementary and Alternative MedicineVolume 2011 2011, Article ID 784826, 10 pages

Original ArticleSchool of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore 632 014, Tamil Nadu, India

Received 27 January 2009; Accepted 8 September 2009

Copyright © 2011 Gunjan Guha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cyanthillium cinereum Less. H. Rob. Asteraceae has been traditionally known for its medicinal properties, all aspects of which are yet to be exploited. This study was aimed at investigating the therapeutic potential of polar methanolic and aqueous and nonpolar hexane and chloroform crude extracts of the whole plant. Several parameters including free-radical DPPH

, ABTS

, H2O2 and

OH scavenging, reducing power, protection of DNA against oxidative damage, cytotoxicity, inhibition of oxidative hemolysis in erythrocytes, total phenolic content and inhibition of lipid peroxidation were examined. All the free-radical generating assay models demonstrated positive scavenging efficiency with differential but considerable magnitudes for the four extracts. However, only the hexane extract showed significant H2O2 scavenging effect. Lipid peroxidation was estimated by thiobarbituric acid-malondialdehyde MDA reaction, and a high degree of inhibition was shown by all the extracts. Reducing power of the polar extracts was higher than the non-polar ones. All extracts showed a concentration-dependent increase in phenolic contents. Oxidative damage to erythrocytes was hindered by all extracts in diverse degrees. XTT assay showed that all extracts have mild cytotoxic property. The aqueous extract evidently demonstrated protective effect on pBR322 plasmid DNA against oxidative breakdown. These results suggested the potential of C. cinereum as medicine against free-radical-associated oxidative damage and related degenerative diseases involving metabolic stress, genotoxicity and cytotoxicity.





Author: Gunjan Guha, V. Rajkumar, R. Ashok Kumar, and Lazar Mathew

Source: https://www.hindawi.com/



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