Differential in vitro inhibitory activity against HIV-1 of alpha-1-3- and alpha-1-6-D-mannose specific plant lectins : Implication for microbicide developmentReport as inadecuate




Differential in vitro inhibitory activity against HIV-1 of alpha-1-3- and alpha-1-6-D-mannose specific plant lectins : Implication for microbicide development - Download this document for free, or read online. Document in PDF available to download.

Journal of Translational Medicine

, 5:28

First Online: 12 June 2007Received: 05 March 2007Accepted: 12 June 2007DOI: 10.1186-1479-5876-5-28

Cite this article as: Saïdi, H., Nasreddine, N., Jenabian, MA. et al. J Transl Med 2007 5: 28. doi:10.1186-1479-5876-5-28

Abstract

BackgroundPlant lectins such as Galanthus nivalis agglutinin GNA and Hippeastrum hybrid agglutinin HHA are natural proteins able to link mannose residues, and therefore inhibit HIV-target cell interactions. Plant lectins are candidate for microbicide development.

ObjectiveTo evaluate the activity against HIV of the mannose-specific plant lectins HHA and GNA at the cellular membrane level of epithelial cells and monocyte-derived dendritic cells MDDC, two potential target cells of HIV at the genital mucosal level.

MethodsThe inhibitory effects of HHA and GNA were evaluated on HIV adsorption to genital epithelial HEC-1A cell line, on HIV transcytosis throughout a monolayer of polarized epithelial HEC-1A cells, on HIV adsorption to MDDC and on transfer of HIV from MDDC to autologous T lymphocytes.

ResultsHHA faintly inhibited attachment to HEC-1A cells of the R5-tropic HIV-1Ba-L strain, in a dose-dependent manner, whereas GNA moderately inhibited HIV adsorption in the same context, but only at high drug doses. Only HHA, but not GNA, inhibited HIV-1JR-CSF transcytosis in a dose-dependent manner. By confocal microscopy, HHA, but not GNA, was adsorbed at the epithelial cell surface, suggesting that HHA interacts specifically with receptors mediating HIV-1 transcytosis. Both plant lectins partially inhibited HIV attachment to MDDC. HHA inhibited more efficiently the transfer of HIV from MDDC to T cell, than GNA. Both HHA and GNA lacked toxicity below 200 μg-ml irrespective the cellular system used and do not disturb the monolayer integrity of epithelial cells.

ConclusionThese observations demonstrate higher inhibitory activities of the lectin plant HHA by comparison to GNA, on HIV adsorption to HEC-1A cell line, HIV transcytosis through HEC-1A cell line monolayer, HIV adsorption to MDDC and HIV transfer from MDDC to T cells, highlighting the potential interest of HHA as effective microbicide against HIV.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-5-28 contains supplementary material, which is available to authorized users.

Hela Saïdi, Nadine Nasreddine contributed equally to this work.

Download fulltext PDF



Author: Hela Saïdi - Nadine Nasreddine - Mohammad-Ali Jenabian - Maxime Lecerf - Dominique Schols - Corinne Krief - Jan Balzarini

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents