Phospholipase C-η2 interacts with nuclear and cytoplasmic LIMK-1 during retinoic acid-stimulated neurite growthReport as inadecuate




Phospholipase C-η2 interacts with nuclear and cytoplasmic LIMK-1 during retinoic acid-stimulated neurite growth - Download this document for free, or read online. Document in PDF available to download.

Histochemistry and Cell Biology

, Volume 145, Issue 2, pp 163–173

First Online: 15 December 2015Accepted: 24 November 2015DOI: 10.1007-s00418-015-1390-7

Cite this article as: Arastoo, M., Hacker, C., Popovics, P. et al. Histochem Cell Biol 2016 145: 163. doi:10.1007-s00418-015-1390-7

Abstract

Neurite growth is central to the formation and differentiation of functional neurons, and recently, an essential role for phospholipase C-η2 PLCη2 in neuritogenesis was revealed. Here we investigate the function of PLCη2 in neuritogenesis using Neuro2A cells, which upon stimulation with retinoic acid differentiate and form neurites. We first investigated the role of the PLCη2 calcium-binding EF-hand domain, a domain that is known to be required for PLCη2 activation. To do this, we quantified neurite outgrowth in Neuro2A cells, stably overexpressing wild-type PLCη2 and D256A EF-hand and H460Q active site PLCη2 mutants. Retinoic acid-induced neuritogenesis was highly dependent on PLCη2 activity, with the H460Q mutant exhibiting a strong dominant-negative effect. Expression of the D256A mutant had little effect on neurite growth relative to the control, suggesting that calcium-directed activation of PLCη2 is not essential to this process. We next investigated which cellular compartments contain endogenous PLCη2 by comparing immunoelectron microscopy signals over control and knockdown cell lines. When signals were analyzed to reveal specific labeling for PLCη2, it was found to be localized predominantly over the nucleus and cytosol. Furthermore in these compartments and also in growing neurites, a proximity ligand assay revealed that PLCη2 specifically interacts with LIMK-1 in Neuro2A cells. Taken together, these data emphasize the importance of the PLCη2 EF-hand domain and articulation of PLCη2 with LIMK-1 in regulating neuritogenesis.

KeywordsCalcium signaling Cell differentiation Electron microscopy Neuritogenesis Protein–protein interaction An erratum to this article can be found at http:-dx.doi.org-10.1007-s00418-016-1418-7.

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Author: Mohammed Arastoo - Christian Hacker - Petra Popovics - John M. Lucocq - Alan J. Stewart

Source: https://link.springer.com/



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