Decreased CD127 Expression on CD4 T-Cells and Elevated Frequencies of CD4 CD25 CD127− T-Cells in Children with Long-Lasting Type 1 DiabetesReport as inadecuate




Decreased CD127 Expression on CD4 T-Cells and Elevated Frequencies of CD4 CD25 CD127− T-Cells in Children with Long-Lasting Type 1 Diabetes - Download this document for free, or read online. Document in PDF available to download.

Clinical and Developmental ImmunologyVolume 2013 2013, Article ID 459210, 11 pages

Research Article

Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland

Department of Allergology and Internal Medicine, University of Bialystok, 15-276 Bialystok, Poland

Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University of Bialystok, 15-274 Bialystok, Poland

Department of Hematological Diagnostics, Medical University of Bialystok, 15-274 Bialystok, Poland

Department of Immunology, Medical University of Bialystok, 15-276 Bialystok, Poland

Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland

Department of Statistics and Medical Informatics, Medical University of Bialystok, 15-295 Bialystok, Poland

Received 24 July 2013; Revised 28 September 2013; Accepted 1 October 2013

Academic Editor: Mario Clerici

Copyright © 2013 Marcin Moniuszko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pathobiology of type 1 diabetes T1D is predominantly associated with T-cell-related actions. Homeostasis of majority of T-cells is critically dependent on signals mediated by CD127 interleukin-7 receptor, IL-7R. In contrast, regulatory T-cells express very little CD127 and thereby may be delineated by CD4+CD25+CD127− phenotype. Here we aimed to analyze CD127 expression on CD4+ and CD8+ T-cells and enumerate CD4+CD25+CD127− T-cells in long-lasting T1D. T-cells were analyzed by flow cytometry and immunologic data were correlated with vascular, metabolic, and inflammatory parameters. We demonstrated significantly decreased CD127 levels on CD4+, but not CD8+, T cells in T1D pediatric patients. Interestingly, frequencies of CD4+CD25+CD127− T-cells were significantly enhanced in T1D children and correlated well with frequencies of CD34+CD144+ endothelial progenitor cells and CD4+CD25− T-cells. Levels of CD127 on both CD4+ and CD8+ T-cells in T1D patients were not correlated to each other or HbA1C. Interestingly, however, CD127 levels on CD4+ T-cells were significantly correlated to frequencies of CD4+CD25+CD127− T-cells, whereas CD127 levels on CD8+ T-cells were significantly correlated to concentrations of VEGF and triglycerides. Our data indicate that CD127 expression is differentially modulated on CD4+ and CD8+ T-cells in the course of T1D. Moreover, we demonstrated that, in contrast to recent-onset T1D, long-lasting T1D is associated with enhancement of T-cells with regulatory phenotype.





Author: Marcin Moniuszko, Barbara Glowinska-Olszewska, Malgorzata Rusak, Marta Jeznach, Kamil Grubczak, Danuta Lipinska, Robert Mile

Source: https://www.hindawi.com/



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