Functional significance of CD105-positive cells in papillary renal cell carcinomaReport as inadecuate




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BMC Cancer

, 17:21

Cell and molecular biology

Abstract

BackgroundCD105 was postulated as a renal cell carcinoma RCC stem cell marker, and CD133 as a putative RCC progenitor. Hypoxia, a natural microenvironment that prevails in tumors, was also incorporated into the study, especially in terms of the promotion of hypothetical stem-like cell properties.

MethodsWithin this study, we verify the existence of CD105+ and CD133+ populations in selected papillary subtype RCC pRCC cell lines. Both populations were analyzed for correlation with stem-like cell properties, such as stemness gene expression, and sphere and colony formation. For the preliminary analysis, several RCC cell lines were chosen 786-O, SMKT-R2, Caki-2, 796-P, ACHN, RCC6 and the control was human kidney cancer stem cells HKCSC and renal cells of embryonic origin ASE-5063. Four cell lines were chosen for further investigation: Caki-2 one of the highest numbers of CD105+ cells; primary origin, ACHN a low number of CD105+ cells; metastatic origin, HKCSC putative positive control, and ASE-5063 additional control.

ResultsIn 769-P and RCC6, we could not detect a CD105+ population. Hypoxia variously affects pRCC cell growth, and mainly diminishes the stem-like properties of cells. Furthermore, we could not observe the correlation of CD105 and-or CD133 expression with the enhancement of stem-like properties.

ConclusionsBased on this analysis, CD105-CD133 cannot be validated as cancer stem cell markers of pRCC cell lines.

KeywordsRenal cell cancer Papillary cancer Cancer stem cells Tumor initiating cells CD105 Endoglin AbbreviationsALDHAldehyde dehydrogenase

ccRCCClear cell renal cell carcinoma

CD105Endoglin

CD133Prominin-1

CD24Cluster of differentiation 24

CD44Indian blood group

CD90Cluster of differentiation 90

chRCCChromophobe renal cell carcinoma

CSCCancer stem cells

CTR2Cooper uptake 2

CXCR4C-X-C chemokine receptor type 4

HIF1Hypoxia-inducible factor 1

HIF2Hypoxia-inducible factor 2

HKCSCHuman kidney cancer stem cells

Oct4Octamer-binding transcription factor 4

PPIAPeptidylprolyl isomerase A

pRCCPapillary renal cell carcinoma

RCCRenal cell carcinoma

Sox2SRY sex determining region Y-box

TFTranscription factor

TGFβTransforming growth factor beta

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Author: Damian Matak - Klaudia K. Brodaczewska - Cezary Szczylik - Irena Koch - Adam Myszczyszyn - Monika Lipiec - Slawomir Lewick

Source: https://link.springer.com/







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