Phylogenetic inferences of Nepenthes species in Peninsular Malaysia revealed by chloroplast trnL intron and nuclear ITS DNA sequencesReport as inadecuate




Phylogenetic inferences of Nepenthes species in Peninsular Malaysia revealed by chloroplast trnL intron and nuclear ITS DNA sequences - Download this document for free, or read online. Document in PDF available to download.

BMC Research Notes

, 10:67

First Online: 26 January 2017Received: 13 June 2016Accepted: 09 January 2017DOI: 10.1186-s13104-017-2379-1

Cite this article as: Bunawan, H., Yen, C.C., Yaakop, S. et al. BMC Res Notes 2017 10: 67. doi:10.1186-s13104-017-2379-1

Abstract

BackgroundThe chloroplastic trnL intron and the nuclear internal transcribed spacer ITS region were sequenced for 11 Nepenthes species recorded in Peninsular Malaysia to examine their phylogenetic relationship and to evaluate the usage of trnL intron and ITS sequences for phylogenetic reconstruction of this genus.

ResultsPhylogeny reconstruction was carried out using neighbor-joining, maximum parsimony and Bayesian analyses. All the trees revealed two major clusters, a lowland group consisting of N. ampullaria, N. mirabilis, N. gracilis and N. rafflesiana, and another containing both intermediately distributed species N. albomarginata and N. benstonei and four highland species N. sanguinea, N. macfarlanei, N. ramispina and N. alba.

ConclusionsThe trnL intron and ITS sequences proved to provide phylogenetic informative characters for deriving a phylogeny of Nepenthes species in Peninsular Malaysia. To our knowledge, this is the first molecular phylogenetic study of Nepenthes species occurring along an altitudinal gradient in Peninsular Malaysia.

KeywordsITS Nepenthes Peninsular Malaysia Pitcher plant AbbreviationsITSinternal transcribed spacer

UKMBUniversiti Kebangsaan Malaysia Herbarium

NJneighbor-joining

MPmaximum parsimony





Author: Hamidun Bunawan - Choong Chee Yen - Salmah Yaakop - Normah Mohd Noor

Source: https://link.springer.com/



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