Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion ProteinReport as inadecuate




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Clinical and Developmental ImmunologyVolume 2011 2011, Article ID 831704, 14 pages

Research Article

Institute of Biochemistry, College of Life Sciences, Zhejiang University Zijingang Campus, 388 Yuhangtang Road, Hangzhou 310058, China

Institute of Biochemistry, College of Life Sciences, Zhejiang Sci-Tech University, Second Avenue, Hangzhou 310018, China

Received 14 January 2011; Revised 15 April 2011; Accepted 30 April 2011

Academic Editor: Nick Giannoukakis

Copyright © 2011 Qiaohong Meng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In animals, oral administration of the cholera toxin B CTB subunit conjugated to the autoantigen insulin enhances the specific immune-unresponsive state. This is called oral tolerance and is capable of suppressing autoimmune type 1 diabetes T1D. However, the process by which the CTB-insulin CTB-INS protein works as a therapy for T1D in vivo remains unclear. Here, we successfully expressed a green fluorescent protein- GFP- tagged CTB-Ins CTB-Ins-GFP fusion protein in silkworms in a pentameric form that retained the native ability to activate the mechanism. Oral administration of the CTB-Ins-GFP protein induced special tolerance, delayed the development of diabetic symptoms, and suppressed T1D onset in nonobese diabetic NOD mice. Moreover, it increased the numbers of CD4

CD25

Foxp3

T regulatory Treg cells in peripheral lymph tissues and affected the biological activity of spleen cells. This study demonstrated that the CTB-Ins-GFP protein produced in silkworms acted as an oral protein vaccine, inducing immunological tolerance involving CD4

CD25

Foxp3

Treg cells in treating T1D.





Author: Qiaohong Meng, Wenfeng Wang, Xiaowen Shi, Yongfeng Jin, and Yaozhou Zhang

Source: https://www.hindawi.com/



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