Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic AcidReport as inadecuate




Comparison of Disk Diffusion and Etest Methods to Determine the Susceptibility of Staphylococcus aureus Circulating in Riyadh, Saudi Arabia to Fusidic Acid - Download this document for free, or read online. Document in PDF available to download.

International Journal of MicrobiologyVolume 2012 2012, Article ID 391251, 4 pages

Research Article

Department of Pathology, Microbiology Unit, College of Medicine, King Saud University and King Khalid University Hospital, Riyadh 11411, Saudi Arabia

Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06510, USA

Pathology and Laboratory Medicine Service, Veteran Affairs VA Connecticut Healthcare System, West Haven, CT 06516, USA

Departments of Pediatrics and Pharmacology, Yale School of Medicine, New Haven, CT 06510, USA

Department of Medical Sciences, The Frank H. Netter MD School of Medicine, Quinnipiac University, Hamden, CT 06518-7908, USA

Received 12 March 2012; Revised 24 May 2012; Accepted 7 June 2012

Academic Editor: John Tagg

Copyright © 2012 Ali M. Somily et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fusidic acid is a common therapy for staphylococcal infections in Saudi Arabia, but reports have suggested high rates of resistance among clinical isolates. Susceptibility testing of S. aureus to fusidic acid is further complicated by the lack of consensus on mean inhibitory concentrations MIC and disk diffusion cutoffs to determine resistance. The purpose of this study was to determine the correlation between disk diffusion and Etest determined MIC susceptibility results in clinical isolates of S. aureus from a large academic hospital in Riyadh, Saudi Arabia. Our data demonstrate excellent correlation between Etest determined MIC and disk diffusion susceptibility data, using either previously proposed zone sizes of ≥21 mm as susceptible and ≤18 mm as resistant or the EUCAST recommended zone size of ≤24 mm for resistance, in an area with relatively high rates of fusidic acid resistance.





Author: Ali M. Somily, David R. Peaper, Elijah Paintsil, and Thomas S. Murray

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents