Smartphone-Based Dual-Modality Imaging System for Quantitative Detection of Color or Fluorescent Lateral Flow Immunochromatographic StripsReport as inadecuate




Smartphone-Based Dual-Modality Imaging System for Quantitative Detection of Color or Fluorescent Lateral Flow Immunochromatographic Strips - Download this document for free, or read online. Document in PDF available to download.

Nanoscale Research Letters

, 12:291

First Online: 21 April 2017Received: 15 March 2017Accepted: 13 April 2017DOI: 10.1186-s11671-017-2078-9

Cite this article as: Hou, Y., Wang, K., Xiao, K. et al. Nanoscale Res Lett 2017 12: 291. doi:10.1186-s11671-017-2078-9

Abstract

Nowadays, lateral flow immunochromatographic assays are increasingly popular as a diagnostic tool for point-of-care POC test based on their simplicity, specificity, and sensitivity. Hence, quantitative detection and pluralistic popular application are urgently needed in medical examination. In this study, a smartphone-based dual-modality imaging system was developed for quantitative detection of color or fluorescent lateral flow test strips, which can be operated anywhere at any time. In this system, the white and ultra-violet UV light of optical device was designed, which was tunable with different strips, and the Sobel operator algorithm was used in the software, which could enhance the identification ability to recognize the test area from the background boundary information. Moreover, this technology based on extraction of the components from RGB format red, green, and blue of color strips or only red format of the fluorescent strips can obviously improve the high-signal intensity and sensitivity. Fifty samples were used to evaluate the accuracy of this system, and the ideal detection limit was calculated separately from detection of human chorionic gonadotropin HCG and carcinoembryonic antigen CEA. The results indicated that smartphone-controlled dual-modality imaging system could provide various POC diagnoses, which becomes a potential technology for developing the next-generation of portable system in the near future.

KeywordsSmartphone Dual-modality imaging Lateral flow strip Quantitative detection 



Author: Yafei Hou - Kan Wang - Kun Xiao - Weijian Qin - Wenting Lu - Wei Tao - Daxiang Cui

Source: https://link.springer.com/







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