Identification of proteins differentially expressed by Chlamydia trachomatis treated with chlamydiaphage capsid protein VP1 during intracellular growthReport as inadecuate




Identification of proteins differentially expressed by Chlamydia trachomatis treated with chlamydiaphage capsid protein VP1 during intracellular growth - Download this document for free, or read online. Document in PDF available to download.

Archives of Microbiology

pp 1–11

First Online: 25 April 2017Received: 01 November 2016Revised: 21 January 2017Accepted: 17 April 2017DOI: 10.1007-s00203-017-1381-2

Cite this article as: Ma, J., Sun, Y., Sun, C. et al. Arch Microbiol 2017. doi:10.1007-s00203-017-1381-2

Abstract

Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases. Our research pertains to the inhibitory effect and molecular mechanism of the chlamydiaphage capsid protein VP1 on the growth of Chlamydia trachomatis. In this research, the capsid protein VP1 of the guinea-pig conjunctivitis chlamydiaphage phiCPG1 was expressed, purified and identified, and then, it was applied to the cultivation of different serovars of Chlamydia trachomatis and Chlamydia psittaci. The inhibitory effect was observed in each serovar of Chlamydia trachomatis D, E, F, G, H, I, K, and L2 and Chlamydia psittaci inoculated with VP1 protein. The inhibition affection of VP1 on the growth of Chlamydia trachomatis was caused by the changes of expressions of some related proteins including 36 proteins up-regulated and 81 proteins down-regulated in the development cycle of Ct through the label-free test, and the transcription levels of these proteins, including Hc1, pmpD, and MOMP, were confirmed by RT-PCR. It provides information that is essential for understanding the mechanism of chlamydiaphage capsid protein VP1 on chlamydia and a new direction for further clinical treatment of chlamydial infection.

KeywordsChlamydia trachomatis Chlamydiaphages VP1 Inhibition Communicated by Shuang-Jiang Liu.





Author: Jingyue Ma - Yina Sun - Changgui Sun - Quan Zhou - Manli Qi - Jie Kong - Jing Wang - Yuanjun Liu - Quanzhong Liu

Source: https://link.springer.com/







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