Population Diversity of Campylobacter jejuni in Poultry and Its Dynamic of Contamination in Chicken MeatReport as inadecuate




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BioMed Research International - Volume 2015 2015, Article ID 859845, 10 pages -

Research ArticleIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise -G.Caporale-, National Reference Laboratory for Campylobacter, 64100 Teramo, Italy

Received 23 July 2014; Revised 27 November 2014; Accepted 30 November 2014

Academic Editor: Miguel Prieto

Copyright © 2015 Francesca Marotta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to analyse the diversity of the Campylobacter jejuni population in broilers and to evaluate the major source of contamination in poultry meat. Eight rearing cycles over one year provided samples from three different broiler farms processed at the same slaughterhouse. A total of 707  C. jejuni were isolated from cloacal swabs before slaughter and from the breast skin of carcasses after slaughter and after chilling. All suspected Campylobacter colonies were identified with PCR assays and C. jejuni was genotyped by sequence analysis of the flaA short variable region SVR and by pulsed-field gel electrophoresis PFGE using SmaI enzyme. Phenotypic antibiotic resistance profiles were also assayed using minimal inhibitory concentration MIC. The flocks carried many major C. jejuni clones possibly carrying over the rearing cycles, but cross contamination between farms may happen. Many isolates were resistant to fluoroquinolones, raising an issue of high public concern. Specific Campylobacter populations could be harboured within each poultry farm, with the ability to contaminate chickens during each new cycle. Thus, although biosecurity measures are applied, with a persistent source of contamination, they cannot be efficient. The role of the environment needs further investigation to better address strategies to control Campylobacter.





Author: Francesca Marotta, Giuliano Garofolo, Guido Di Donato, Giuseppe Aprea, Ilenia Platone, Silvia Cianciavicchia, Alessandra Ale

Source: https://www.hindawi.com/



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