Inflammation Related MicroRNAs Are Modulated in Total Plasma and in Extracellular Vesicles from Rats with Chronic Ingestion of SucroseReport as inadecuate




Inflammation Related MicroRNAs Are Modulated in Total Plasma and in Extracellular Vesicles from Rats with Chronic Ingestion of Sucrose - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2016 2016, Article ID 2489479, 7 pages -

Research Article

Posgrado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, Iztapalapa, 09340 Mexico City, Mexico

Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología -Ignacio Chávez-, Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico

Departamento de Inmunología, Instituto Nacional de Cardiología -Ignacio Chávez-, Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico

Colegio de Ciencias y Humanidades, Universidad Autónoma de la Ciudad de México-San Lorenzo Tezonco, Av. Prolongación San Isidro No. 151, Col. San Lorenzo Tezonco, Iztapalapa, 09790 Mexico City, Mexico

Departamento de Endocrinología, Instituto Nacional de Cardiología -Ignacio Chávez-, Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico

Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Xochimilco, Calzada del Hueso 1100, Villa Quietud, Coyoacan, 04960 Mexico City, Mexico

Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Tlalpan, 14610 Mexico City, Mexico

Laboratorio de Farmacología y Toxicología, Hospital Infantil de México Federico Gómez, Calle Dr. Márquez No. 162, Cuauhtemoc, Doctores, 06720 Ciudad de México, Mexico

Received 1 July 2016; Revised 18 October 2016; Accepted 31 October 2016

Academic Editor: Yang Zhou

Copyright © 2016 Malinalli Brianza-Padilla et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Circulating microRNAs miRNAs and the functional implications of miRNAs contained in extracellular vesicles EVs have gained attention in the last decade. Little is known about the regulation of the abundance of plasma miRNAs in response to chronic ingestion of carbohydrates. Therefore, we explored the circulating levels of miR-21, miR-146a, miR-155, and miR-223 in rats consuming sucrose in drinking water. Weanling Wistar rats were 25 weeks with 30% sucrose in drinking water, and miRNAs expression was determined in total plasma and in microvesicles, by RT-qPCR with TaqMan probe based assays for miR-21, miR-146a, miR-155, and miR-223, using cel-miR-39 as spike in control and reference. Endotoxemia was also measured. Sucrose-fed animals showed higher body weight and retroperitoneal adipose tissue as well as higher glucose and triglyceride plasma levels than controls. Plasma endotoxin levels were low and not different among groups. Plasma miR-21 and miR-223 were higher in the sucrose group , whereas miR-155 tended to be lower , and miR-146a did not show significant differences. In the plasma EVs the same trend was found except for miR-146a that showed significantly higher levels . Overall, our results show that high carbohydrate ingestion modulates circulating miRNAs levels related to an inflammatory response.





Author: Malinalli Brianza-Padilla, Roxana Carbó, Julio C. Arana, Gonzalo Vázquez-Palacios, Martha A. Ballinas-Verdugo, Guillermo C.

Source: https://www.hindawi.com/



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