Serial Analysis of Gene Expression: Applications in Human StudiesReport as inadecuate




Serial Analysis of Gene Expression: Applications in Human Studies - Download this document for free, or read online. Document in PDF available to download.

Journal of Biomedicine and Biotechnology - Volume 2004 2004, Issue 2, Pages 113-120

Review article International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India

Received 7 August 2003; Accepted 13 November 2003

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Serial analysis of gene expression SAGE is a powerful tool,which provides quantitative and comprehensive expression profileof genes in a given cell population. It works by isolating shortfragments of genetic information from the expressed genes that arepresent in the cell being studied. These short sequences, calledSAGE tags, are linked together for efficient sequencing. Thefrequency of each SAGE tag in the cloned multimers directlyreflects the transcript abundance. Therefore, SAGE results in anaccurate picture of gene expression at both the qualitative andthe quantitative levels. It does not require a hybridizationprobe for each transcript and allows new genes to be discovered.This technique has been applied widely in human studies andvarious SAGE tags-SAGE libraries have been generated fromdifferent cells-tissues such as dendritic cells, lung fibroblastcells, oocytes, thyroid tissue, B-cell lymphoma, culturedkeratinocytes, muscles, brain tissues, sciatic nerve, culturedSchwann cells, cord blood-derived mast cells, retina, macula,retinal pigment epithelial cells, skin cells, and so forth. Inthis review we present the updated information on theapplications of SAGE technology mainly to human studies.





Author: Renu Tuteja and Narendra Tuteja

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents