Neuroprotective effect of salvianolate lyophilized injection against cerebral ischemia in type 1 diabetic ratsReport as inadecuate




Neuroprotective effect of salvianolate lyophilized injection against cerebral ischemia in type 1 diabetic rats - Download this document for free, or read online. Document in PDF available to download.

BMC Complementary and Alternative Medicine

, 17:258

First Online: 10 May 2017Received: 20 December 2016Accepted: 11 April 2017DOI: 10.1186-s12906-017-1738-8

Cite this article as: Wang, F., He, Q., Wang, J. et al. BMC Complement Altern Med 2017 17: 258. doi:10.1186-s12906-017-1738-8

Abstract

BackgroundSalvianolate lyophilized injection SLI has been clinically used in China for the treatment of acutely cerebral infarction. Clinical and experimental studies have shown that Diabetes mellitus DM not only increases the risk of ischemic stroke recurrence but also leads to poor outcomes and increases fatality rates after stroke. Our previous study has proved that SLI can reduce the infarct volume after stroke in type 1 diabetic rats. The aim of the study is to explore the mechanism of SLI on stroke outcome in type 1 diabetic T1DM rats.

MethodsType 1 diabetes rats model T1DM was induced in male Wistar rats by intraperitoneal i.p injection of streptozotocin 60 mg-kg and T1DM rats were subjected to intraluminal middle cerebral artery occlusion MCAO. The T1DM + MCAO rats were randomly divided into six groups: sham-operated, model-vehicle, positive control group Edaravone-treating, DE 6 mg-kg and SLI-treating group 10.5 mg-kg, 21 mg-kg and 42 mg-kg. SLI and DE were administered by tail vein injection at 3 h after MCAO, then daily for 14 days. Micro-CT scans of the brain tissue revealed vessel characteristics and distribution in the ischemia zone. Glucose uptake was analyzed by PET-CT. RAGE, MMP9 and inflammatory factors COX-2, TNF-α and ICAM-1, HQ-1, HQO-1 and Nrf-2 expression levels in the ischemic brain tissue were analyzed by Immunofluorescence staining and Western blot at 14 days after MCAO.

ResultsIn this study, we have demonstrated that SLI treatment significantly increased the number of brain microvasculature in ipsilateral and glucose uptake in cortex, hippocampus and penumbra in the T1DM + MCAO rats. SLI also significantly decreased the expression of RAGE, MMP9 and inflammatory factors expression, and increased the expression of HQ-1, HQO-1 and Nrf-2 in T1DM + MCAO rats.

ConclusionThe study showed that SLI could protect against cerebral ischemia injury in T1DM + MCAO rats and the mechanism is related to decrease inflammatory factors and activate of the Nrf2-HO-1 signaling pathway.

KeywordsStroke Diabetes Salvianolate lyophilized injection Nrf2-HO-1 pathway AbbreviationsAGEsAdvanced Glycation End Products

ANOVAOne-way analysis of variance

CCACommon carotid artery

Conn.DConnectivity Density

COX-2Cyclo-oxyge-nase-2

DEEdaravone

ECAExternal carotid artery

HO-1Heme-oxygenase-1

HQO-1NADPH quinine oxidoreductase

ICAInternal carotid artery

ICAM1Intercellular cell adhesion molecule-1

LALithospermic acid

M,ThMean Vascular Thickness

M.SpMean Vascular Separation

MCAOEmbolic middle cerebral artery occlusion

MMP9Matrix metallopeptidases 9

Nrf2Nuclear factor erythroid 2-related factor 2

RARosmarinic acid

SalBSalvianolic acid B

SLISalvianolate lyophilized injection

STZStreptozotocin

T1DMType 1 diabetic

TCMTraditional Chinese Medicine

TNF-\ \boldsymbol{\upalpha} \Tumor necrosis factor-\ \boldsymbol{\upalpha} \

VVFVascular Volume Fraction





Author: Fujiang Wang - Qiansong He - Jinxin Wang - Qing Yuan - Hong Guo - Lijuan Chai - Shaoxia Wang - Limin Hu - Yue Zhang

Source: https://link.springer.com/







Related documents