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BioMed Research InternationalVolume 2015 2015, Article ID 578764, 6 pages

Research Article

Children’s Hospital of Zhengzhou, Zhengzhou 450053, China

School of Life Science and Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China

Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA

Received 17 October 2015; Revised 10 November 2015; Accepted 15 November 2015

Academic Editor: Lucia Lopalco

Copyright © 2015 Wen-Ping Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Antibacterial and antifungal peptides have increasingly been used to combat the antibiotic-resistant microbes in recent years. KW-13, a novel cationic α-helical antibacterial peptide consisting of 13 amino acid residues, was designed and chemically synthesized. The peptide has a net charge of +6 with a total hydrophobic ratio of 38%. The antibacterial experiments revealed that KW-13 strongly inhibited the growth of human pathogenic bacteria with minimal inhibitory concentrations of 4 and 16 μg-mL for Staphylococcus epidermidis and Staphylococcus aureus, respectively, while the hemolytic assay showed that this peptide did not destroy human red blood cells in vitro. Scanning electron microscopy imaging of Escherichia coli confirmed that KW-13 can damage the membrane of bacterial cells. Thus, this peptide could be a potential candidate for the treatment of infectious diseases.





Author: Wen-Ping Liu, Ya-Hui Chen, Xin Ming, and Yi Kong

Source: https://www.hindawi.com/



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