Poly- -Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental ColitisReport as inadecuate




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Mediators of InflammationVolume 2013 2013, Article ID 982383, 8 pages

Research Article

Research Division Emerging Innovative Technology, Korea Food Research Institute, 516 Baekhyun-Dong, Bundang-Ku, Seongnam Gyeonggi 463-746, Republic of Korea

University of Science & Technology, Daejeon 305-350, Republic of Korea

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road Qingdao, Shandong 266071, China

Received 10 January 2013; Revised 10 April 2013; Accepted 29 April 2013

Academic Editor: Julio Galvez

Copyright © 2013 Munkhtugs Davaatseren et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Poly-γ-glutamic acid γ-PGA, naturally secreted from various strains of Bacillus, has anti-inflammatory activity. In inflammatory bowel disease IBD, inflammation is promoted and sustained by angiogenesis; however, the role played by γ-PGA in this condition is unclear. Therefore, we evaluated γ-PGA effects on angiogenesis and inflammation in a dextran sulfate sodium- DSS- induced mouse colitis model. Experimental colitis was induced in male C57BL-6 mice by administering 3% DSS. Disease activity index DAI, histopathological scores, microvascular density, myeloperoxidase activity, and VEGF-A and VEGFR2 expression were compared among control mice, DSS-treated mice, and mice receiving 3% DSS along with γ-PGA at 50 mg-kg body weight per day or 3% DSS with γ-PGA at 200 mg-kg body weight per day. We found that γ-PGA significantly attenuated weight loss, DAI, and colon shortening. γ-PGA also significantly reduced histopathological evidence of injury. Moreover, γ-PGA significantly attenuated DSS-induced blood vessel densities. Furthermore, γ-PGA attenuated DSS-induced expression of VEGF-A and its receptor, VEGFR2. In addition, γ-PGA treatment led to reduced recruitment of leukocytes to the inflamed colon. Therefore, our results indicate that γ-PGA has potential application in conditions marked by inflammatory-driven angiogenesis and mucosal inflammation.





Author: Munkhtugs Davaatseren, Jin-Taek Hwang, Jae Ho Park, Myung-Sunny Kim, Shuaiyu Wang, and Mi Jeong Sung

Source: https://www.hindawi.com/



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