Global and site-specific changes in 5-methylcytosine and 5-hydroxymethylcytosine after extended post-mortem intervalReport as inadecuate




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Journal Title:

Frontiers in Genetics

Volume:

Volume 7

Publisher:

Frontiers Media | 2016-06-23, Pages 120-120

Type of Work:

Article | Final Publisher PDF

Abstract: There has been a growing interest in the study of epigenetic mechanisms to elucidate the molecular bases of human brain-related diseases anddisorders. Frequently, researchers utilize post-mortem tissue with the assumption that post-mortem tissue decay has little or no effect on epigenetic marks. Although previous studies show no effect of post-mortem interval on certain epigenetic marks, no such research has been performed on cytosine modifications. In this study, we use DNA from the brains of adult Sprague Dawley rats subjected to post-mortem intervals at room temperature, ranging from 0 to 96 h, to assess the stability of cytosine modifications, namely 5-methycytosine and 5-hydroxymethylcytosine. Our results indicate that neither global nor site-specific levels of 5-methycytosine and 5-hydroxymethylcytosine are affected by the post-mortem intervals we studied. As such, the use of post-mortem tissue to study cytosinemodifications in the context of neurological or neuropsychiatric disorders is appropriate.

Subjects: Biology, Genetics - Health Sciences, Pathology - Research Funding: This work was supported by grants to GT from the Canadian Institutes of Health Research MOP93775, MOP11260, MOP119429, and MOP119430; from the National Institutes of Health 1R01DA033684-01; and by the Fonds de Recherche du Québec – Santé through a Chercheur National salary award and through the Quebec Network on Suicide, Mood Disorders, and Related Disorders.

JG is supported by a CIHR Frederick Banting and Charles Best Doctoral fellowship and CN is supported by the Fonds de Recherche du Québec – Santé Doctoral award.

Keywords: 5-hydroxymethylcytosine - 5-methylcytosine - epigenetics - post-mortem interval - stability -



Author: Jeffrey A. Gross, Corina Nagy, Li Lin, Éic Bonneil, Marissa Maheu, Pierre Thibault, Naguib Mechawar, Peng Jin, Gustavo Turecki,

Source: https://open.library.emory.edu/



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