Do-it-yourself in vitro vasculature that recapitulates in vivo geometries for investigating endothelial-blood cell interactionsReport as inadecuate




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Journal Title:

Scientific Reports

Volume:

Volume 5

Publisher:

Nature Publishing Group: Open Access Journals - Option C | 2015-07-23, Pages 12401-12401

Type of Work:

Article | Final Publisher PDF

Abstract: Investigating biophysical cellular interactions in the circulation currently requires choosing between in vivo models, which are difficult to interpret due in part to the hemodynamic and geometric complexities of the vasculature; or in vitro systems, which suffer from non-physiologic assumptions and-or require specialized microfabrication facilities and expertise. To bridge that gap, we developed an in vitro do-it-yourself perfusable vasculature model that recapitulates in vivo geometries, such as aneurysms, stenoses, and bifurcations, and supports endothelial cell culture. These inexpensive, disposable devices can be created rapidly <2 hours with high precision and repeatability, using standard off-the-shelf laboratory supplies. Using these endothelialized systems, we demonstrate that spatial variation in vascular cell adhesion molecule VCAM-1 expression correlates with the wall shear stress patterns of vascular geometries. We further observe that the presence of endothelial cells in stenoses reduces platelet adhesion but increases sickle cell disease SCD red blood cell RBC adhesion in bifurcations. Overall, our method enables researchers from all disciplines to study cellular interactions in physiologically relevant, yet simple-to-make, in vitro vasculature models.

Keywords: Science and Technology - Multidisciplinary Sciences - Science and Technology - Other Topics - WALL SHEAR-STRESS - SICKLE-CELL - POSTCAPILLARY VENULES - PLATELET-AGGREGATION - SPATIAL GRADIENTS - CEREBRAL ANEURYSM - DISTURBED FLOW - DISEASE - ERYTHROCYTES - BIFURCATION -



Author: Roger G. Mannino, David Myers, Byungwook Ahn, Yichen Wang, Margo Rollins, Hope Gole, Angela S. Lin, Robert Guldberg, Don Giddens,

Source: https://open.library.emory.edu/



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