Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane ModelReport as inadecuate

Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model - Download this document for free, or read online. Document in PDF available to download.

Stem Cells International - Volume 2015 2015, Article ID 257019, 11 pages -

Research Article

Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia

Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Saudi Electronic University, Saudi Arabia

Bone and Joint Research Group, Centre for Human Development Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Science, University of Southampton, Southampton, UK

KMEB, Department of Endocrinology, University of Southern Denmark, Odense, Denmark

Received 5 May 2015; Revised 9 June 2015; Accepted 10 June 2015

Academic Editor: Tong-Chuan He

Copyright © 2015 Radhakrishnan Vishnubalaji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells hNSSCs as being able to differentiate into adipocytes and osteoblasts and potentially other cell types. Recently, we demonstrated that hNSSCs play a role during in vitro angiogenesis and appear to possess a capacity to differentiate into endothelial-like cells; however, their angiogenic potential within an ex vivo environment remains unclear. Current study shows hNSSCs to display significant migration potential in the undifferentiated state and high responsiveness in the in vitro wound healing scratch assay. When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor hVWF, CD31, smooth muscle actin SMA, and factor XIIIa positive cells were observed in the chick endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a higher number of blood vessels containing hNSSCs compared to CAMs transplanted with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5-6, CK19, FXIIIa, and S-100 cells, which warrant further investigation. Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

Author: Radhakrishnan Vishnubalaji, Muhammad Atteya, May Al-Nbaheen, Richard O. C. Oreffo, Abdullah Aldahmash, and Nehad M. Alajez



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