Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute ColitisReport as inadecuate




Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis - Download this document for free, or read online. Document in PDF available to download.

Mediators of Inflammation - Volume 2014 2014, Article ID 837107, 14 pages -

Research Article

Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan

School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan

Department of Primary Care Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan

Received 7 January 2014; Revised 21 March 2014; Accepted 7 April 2014; Published 7 May 2014

Academic Editor: H. Barbaros Oral

Copyright © 2014 Yu-Chen Hou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Migration of T cells into the colon plays a major role in the pathogenesis in inflammatory bowel disease. This study investigated the effects of glutamine Gln supplementation on chemokine receptors and adhesion molecules expressed by T cells in mice with dextran sulfate sodium- DSS- induced colitis. Methods. C57BL-6 mice were fed either a standard diet or a Gln diet replacing 25% of the total nitrogen. After being fed the diets for 5 days, half of the mice from both groups were given 1.5% DSS in drinking water to induce colitis. Mice were killed after 5 days of DSS exposure. Results. DSS colitis resulted in higher expression levels of P-selectin glycoprotein ligand- PSGL- 1, leukocyte function-associated antigen- LFA- 1, and C-C chemokine receptor type 9 CCR9 by T helper Th and cytotoxic T Tc cells, and mRNA levels of endothelial adhesion molecules in colons were upregulated. Gln supplementation decreased expressions of PSGL-1, LFA-1, and CCR9 by Th cells. Colonic gene expressions of endothelial adhesion molecules were also lower in Gln-colitis mice. Histological finding showed that colon infiltrating Th cells were less in the DSS group with Gln administration. Conclusions. Gln supplementation may ameliorate the inflammation of colitis possibly via suppression of T cell migration.





Author: Yu-Chen Hou, Jin-Ming Wu, Ming-Yang Wang, Ming-Hsun Wu, Kuen-Yuan Chen, Sung-Ling Yeh, and Ming-Tsan Lin

Source: https://www.hindawi.com/



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