Simultaneous Determination and Pharmacokinetic Study of Quercetin, Luteolin, and Apigenin in Rat Plasma after Oral Administration of Matricaria chamomilla L. Extract by HPLC-UVReport as inadecuate




Simultaneous Determination and Pharmacokinetic Study of Quercetin, Luteolin, and Apigenin in Rat Plasma after Oral Administration of Matricaria chamomilla L. Extract by HPLC-UV - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative Medicine - Volume 2017 2017, Article ID 8370584, 7 pages - https:-doi.org-10.1155-2017-8370584

Research Article

Beijing University of Chinese Medicine, Beijing 100029, China

Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China

Correspondence should be addressed to Jian Ni

Received 21 January 2017; Accepted 20 February 2017; Published 8 March 2017

Academic Editor: Jose M. Prieto

Copyright © 2017 Xiaoxv Dong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple and sensitive HPLC-UV method has been developed for the simultaneous determination of quercetin, luteolin, and apigenin in rat plasma after oral administration of Matricaria chamomilla L. extract. The flow rate was set at 1.0 ml-min and the detection wavelength was kept at 350 nm. The calibration curves were linear in the range of 0.11–11.36 μg-ml for quercetin, 0.11–11.20 μg-ml for luteolin, and 0.11–10.60 μg-ml for apigenin, respectively. The intraday and interday precisions RSD were less than 8.32 and 8.81%, respectively. The lower limits of quantification LLOQ of the three compounds were 0.11 μg-ml. The mean recoveries for quercetin, luteolin, and apigenin were 99.11, 95.62, and 95.21%, respectively. Stability studies demonstrated that the three compounds were stable in the preparation and analytical process. The maximum plasma concentration was 0.29 ± 0.06, 3.04 ± 0.60, and 0.42 ± 0.10 μg-ml, respectively. The time to reach the maximum plasma concentration was 0.79 ± 0.25, 0.42 ± 0.09, and 0.51 ± 0.13 h, respectively. The validated method was successfully applied to investigate the pharmacokinetics study of quercetin, luteolin, and apigenin in rat plasma after oral administration of M. chamomilla extract.





Author: Xiaoxv Dong, Wei Lan, Xingbin Yin, Chunjing Yang, Wenping Wang, and Jian Ni

Source: https://www.hindawi.com/



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