Niche matters: The comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulusReport as inadecuate




Niche matters: The comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulus - Download this document for free, or read online. Document in PDF available to download.

Objective

Intrinsic inflammatory characteristics play a pivotal role in stem cell recruitment and homing through migration where the subsequent change in niche has been shown to alter these characteristics. The bone marrow mesenchymal stem cells bmMSCs have been demonstrated to migrate to the endometrium contributing to the stem cell reservoir and regeneration of endometrial tissue. Thus, the aim of the present study was to compare the inflammation-driven migration and cytokine secretion profile of human bmMSCs to endometrial mesenchymal stem cells eMSCs and endometrial fibroblasts eSFs.

Materials and methods

The bmMSCs were isolated from bone marrow aspirates through culturing, whereas eMSCs and eSFs were FACS-isolated. All cell types were tested for their surface marker, proliferation profiles and migration properties towards serum and inflammatory attractants. The cytokine-chemokine secretion profile of 35 targets was analysed in each cell type at basal level along with lipopolysaccharide LPS-induced state.

Results

Both stem cell types, bmMSCs and eMSCs, presented with similar stem cell surface marker profiles as well as possessed high proliferation and migration potential compared to eSFs. In multiplex assays, the secretion of 16 cytokine targets was detected and LPS stimulation expanded the cytokine secretion pattern by triggering the secretion of several targets. The bmMSCs exhibited higher cytokine secretion of vascular endothelial growth factor VEGF-A, stromal cell-derived factor-1 alpha SDF-1α, interleukin-1 receptor antagonist IL-1RA, IL-6, interferon-gamma inducible protein IP-10, monocyte chemoattractant protein MCP-1, macrophage inflammatory protein MIP1α and RANTES compared to eMSCs and-or eSFs after stimulation with LPS. The basal IL-8 secretion was higher in both endometrial cell types compared to bmMSCs.

Conclusion

Our results highlight that similar to bmMSCs, the eMSCs possess high migration activity while the differentiation process towards stromal fibroblasts seemed to result in loss of stem cell surface markers, minimal migration activity and a subtler cytokine profile likely contributing to normal endometrial function.



Author: Masuma Khatun, Anna Sorjamaa, Marika Kangasniemi, Meeri Sutinen, Tuula Salo, Annikki Liakka, Petri Lehenkari, Juha S. Tapanainen,

Source: http://plos.srce.hr/



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