The lack of BTK does not impair monocytes and polymorphonuclear cells functions in X-linked agammaglobulinemia under treatment with intravenous immunoglobulin replacementReport as inadecuate




The lack of BTK does not impair monocytes and polymorphonuclear cells functions in X-linked agammaglobulinemia under treatment with intravenous immunoglobulin replacement - Download this document for free, or read online. Document in PDF available to download.

The lack of BTK in X-linked agammaglobulinemia XLA patients does not affect monocytes and polymorphonuclear cells PMN phenotype and functions. In this study, we show that XLA patients had an increased frequency of the intermediate monocytes subset and that BTK-deficient monocytes and PMN had a normal expression of receptors involved in the activation and cellular responses. We demonstrate that BTK is not required for migration, phagocytosis and the production of reactive oxygen species ROS following engagement of FC gamma receptors FcγR. XLA monocytes and PMN showed an efficient calcium Ca2+-independent activation of oxidative burst, suggesting that oxidative burst is less dependent by Ca2+ mobilization. The phagocytosis was functional and it remained unaltered also after Ca2+ chelation, confirming the independence of phagocytosis on Ca2+ mobilization. Intravenous immunoglobulin IVIg infusion exerted an anti-inflammatory effect by reducing the frequency of pro-inflammatory monocytes. In monocytes, the IVIg reduce the oxidative burst and phagocytosis even if these functions remained efficient.



Author: Filomena Monica Cavaliere , Alessandro Prezzo , Caterina Bilotta, Metello Iacobini, Isabella Quinti

Source: http://plos.srce.hr/



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