KIAA1199 promotes migration and invasion by Wnt-β-catenin pathway and MMPs mediated EMT progression and serves as a poor prognosis marker in gastric cancerReport as inadecuate




KIAA1199 promotes migration and invasion by Wnt-β-catenin pathway and MMPs mediated EMT progression and serves as a poor prognosis marker in gastric cancer - Download this document for free, or read online. Document in PDF available to download.

Background

KIAA1199 was upregulated in diverse cancers, but the association of KIAA1199 with gastric cancer GC, the biological role of KIAA1199 in GC cells and the related molecular mechanisms remain to be elucidated.

Methods

KIAA1199 expression was analysed by reverse transcription-polymerase chain reaction assay RT-PCR and immunohistochemistry IHC in GC patient tissue. The small hairpin RNA shRNA was applied for the knockdown of endogenous KIAA1199 in NCI-N87 and AGS cells. MTT, colony formation, scratch wounding migration, transwell chamber migration and invasion assays were employed respectively to investigate the role of KIAA1199 in GC cells. The potential signaling pathway of KIAA1199 induced migration and invasion was detected.

Results

KIAA1199 was upregulated in GC tissue and was an essential independent marker for poor prognosis. Knockdown KIAA1199 suppressed the proliferation, migration and invasion in GC cells. KIAA1199 stimulated the Wnt-β-catenin signaling pathway and the enzymatic activity of matrix metalloproteinase MMP family members and thus accelerated the epithelial-to-mesenchymal transition EMT progression in GC cells.

Conclusion

These findings demonstrated that KIAA1199 was upregulated in GC tissue and associated with worse clinical outcomes in GC, and KIAA1199 acted as an oncogene by promoting migration and invasion through the enhancement of Wnt-β-catenin signaling pathway and MMPs mediated EMT progression in GC cells.



Author: Shuqin Jia , Tingting Qu , Xiaohong Wang, Mengmeng Feng, Yang Yang, Xuemin Feng, Ruiting Ma, Wenmei Li, Ying Hu, Yi Feng, Ke Ji,

Source: http://plos.srce.hr/



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