Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysatesReport as inadecuate




Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates - Download this document for free, or read online. Document in PDF available to download.

The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 FGFR4 biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers HOOC-MBs and amperometric detection at disposable carbon screen-printed electrodes SPCEs. The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.



Author: Rebeca M. Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Susana Campuzano , María Pedrero, Meryem Farchado, Eva Vargas,

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents