Effects upon metabolic pathways and energy production by SbIII and AsIII-SbIII-oxidase gene aioA in Agrobacterium tumefaciens GW4Report as inadecuate




Effects upon metabolic pathways and energy production by SbIII and AsIII-SbIII-oxidase gene aioA in Agrobacterium tumefaciens GW4 - Download this document for free, or read online. Document in PDF available to download.

Agrobacterium tumefaciens GW4 is a heterotrophic arsenite AsIII-antimonite SbIII-oxidizing strain. The AsIII oxidase AioAB is responsible for AsIII oxidation in the periplasm and it is also involved in SbIII oxidation in Agrobacterium tumefaciens 5A. In addition, SbIII oxidase AnoA and cellular H2O2 are also responsible for SbIII oxidation in strain GW4. However, the deletion of aioA increased the SbIII oxidation efficiency in strain GW4. In the present study, we found that the cell mobility to SbIII, ATP and NADH contents and heat release were also increased by SbIII and more significantly in the aioA mutant. Proteomics and transcriptional analyses showed that proteins-genes involved in SbIII oxidation and resistance, stress responses, carbon metabolism, cell mobility, phosphonate and phosphinate metabolism, and amino acid and nucleotide metabolism were induced by SbIII and were more significantly induced in the aioA mutant. The results suggested that SbIII oxidation may produce energy. In addition, without periplasmic AioAB, more SbIII would enter bacterial cells, however, the cytoplasmic AnoA and the oxidative stress response proteins were significantly up-regulated, which may contribute to the increased SbIII oxidation efficiency. Moreover, the carbon metabolism was also activated to generate more energy against SbIII stress. The generated energy may be used in Sb transportation, DNA repair, amino acid synthesis, and cell mobility, and may be released in the form of heat.



Author: Jingxin Li, Birong Yang, Manman Shi, Kai Yuan, Wei Guo, Mingshun Li, Gejiao Wang

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents