The best of both worlds: A combined approach for analyzing microalgal diversity via metabarcoding and morphology-based methodsReport as inadecuate




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An increasing number of studies use next generation sequencing NGS to analyze complex communities, but is the method sensitive enough when it comes to identification and quantification of species? We compared NGS with morphology-based identification methods in an analysis of microalgal periphyton communities. We conducted a mesocosm experiment in which we allowed two benthic grazer species to feed upon benthic biofilms, which resulted in altered periphyton communities. Morphology-based identification and 454 Roche pyrosequencing of the V4 region in the small ribosomal unit 18S rDNA gene were used to investigate the community change caused by grazing. Both the NGS-based data and the morphology-based method detected a marked shift in the biofilm composition, though the two methods varied strongly in their abilities to detect and quantify specific taxa, and neither method was able to detect all species in the biofilms. For quantitative analysis, we therefore recommend using both metabarcoding and microscopic identification when assessing the community composition of eukaryotic microorganisms.



Author: Sophie Groendahl , Maria Kahlert, Patrick Fink

Source: http://plos.srce.hr/



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