Modulation of Steroidogenic Pathway in Rat Granulosa Cells with Subclinical Cd Exposure and Insulin Resistance: An Impact on Female FertilityReport as inadecuate




Modulation of Steroidogenic Pathway in Rat Granulosa Cells with Subclinical Cd Exposure and Insulin Resistance: An Impact on Female Fertility - Download this document for free, or read online. Document in PDF available to download.

BioMed Research InternationalVolume 2014 2014, Article ID 460251, 13 pages

Research Article

Department of Biochemistry, Faculty of Science, The M. S. University of Baroda, Vadodara, Gujarat 390 002, India

Department of Zoology, Faculty of Science, The M. S. University of Baroda, Vadodara, Gujarat 390 002, India

Gupta’s Pathology Laboratory, Vadodara, Gujarat 390 002, India

Received 22 February 2014; Revised 11 July 2014; Accepted 28 July 2014; Published 19 August 2014

Academic Editor: Leon Spicer

Copyright © 2014 Muskaan Belani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Changes in lifestyle lead to insulin resistance IR in females ultimately predisposing them towards infertility. In addition, cadmium Cd, an environmental endocrine disruptor, is reported for detrimental effects on granulosa cells, thus leading to ovarian dysfunction. A combination of these factors, lifestyle and environment, seems to play a role in etiology of idiopathic infertility that accounts for 50% amongst the total infertility cases. To address this issue, we made an attempt to investigate the extent of Cd impact on insulin-resistant IR granulosa cells. We exposed adult female Charles Foster rats to dexamethasone and confirmed IR condition by fasting insulin resistance index FIRI. On treatment of IR rats with Cd, the preliminary studies demonstrated prolonged estrous cyclicity, decrease in serum estradiol concentrations, abnormal histology of ovary, and increased granulosa cell death. Further gene and protein expression studies of steroidogenic acute regulatory StAR protein, 17β-hydroxysteroid dehydrogenase 17β-HSD, and cytochrome P450 aromatase CYP19A1 were performed. Protein expression studies demonstrated significant decrease in treated groups when compared with control. Study revealed that, in spite of the molecular parameters being affected at varied level, overall ovarian physiology is maximally affected in IR and Cd coexposed group, thus mimicking the condition similar to those prevailing in infertile females.





Author: Muskaan Belani, Nupur Purohit, Prakash Pillai, Sharad Gupta, and Sarita Gupta

Source: https://www.hindawi.com/



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