Diagnostic Accuracy of GeneXpert MTB-RIF Assay in Comparison to Conventional Drug Susceptibility Testing Method for the Diagnosis of Multidrug-Resistant TuberculosisReport as inadecuate




Diagnostic Accuracy of GeneXpert MTB-RIF Assay in Comparison to Conventional Drug Susceptibility Testing Method for the Diagnosis of Multidrug-Resistant Tuberculosis - Download this document for free, or read online. Document in PDF available to download.

Xpert MTB-RIF assay is regarded as a great achievement of modern medicine for the rapid diagnosis of multidrug-resistant tuberculosis MDR-TB. The main purpose of this study was to determine the performance of Xpert MTB-RIF assay compared to conventional drug susceptibility testing DST method for the diagnosis of MDR-TB. A comparative cross sectional study was carried out at German-Nepal Tuberculosis Project, Kathmandu, Nepal, from April 2014 to September 2014. A total of 88 culture positive clinical samples 83 pulmonary and 5 extra-pulmonary received during the study period were analyzed for detection of multidrug-resistant tuberculosis by both GeneXpert MTB-RIF assay and conventional DST method. McNemar chi square test was used to compare the performance of Xpert with that of DST method. A p-value of less than 0.05 was considered as statistically significant. Of total 88 culture positive samples, one was reported as invalid while 2 were found to contain nontuberculous Mycobacteria NTM. Among remaining 85 Mycobacterium tuberculosis culture positive samples, 69 were found to be MDR-TB positive by both methods. The overall sensitivity, specificity, positive predictive value PPV and negative predictive value NPV of GeneXpert MTB-RIF assay were found to be 98.6%, 100%, 100% and 93.8% respectively. Statistically, there was no significant difference between the diagnostic performance of Xpert and conventional DST method for detection of MDR-TB. GeneXpert MTB-RIF assay was found to be highly sensitive, specific and comparable to gold standard conventional DST method for the diagnosis of MDR-TB.



Author: Pratikshya Pandey, Narayan Dutt Pant , Komal Raj Rijal, Bhawana Shrestha, Sirita Kattel, Megha Raj Banjara, Bhagwan Maharjan, Raj

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents