Deletion of ssnA Attenuates the Pathogenicity of Streptococcus suis and Confers Protection against Serovar 2 Strain ChallengeReport as inadecuate




Deletion of ssnA Attenuates the Pathogenicity of Streptococcus suis and Confers Protection against Serovar 2 Strain Challenge - Download this document for free, or read online. Document in PDF available to download.

Streptococcus suis serotype 2 SS2 is a major porcine and human pathogen which causes arthritis, meningitis, and septicemia. Streptococcus suis nuclease A SsnA is a recently discovered deoxyribonuclease DNase, which has been demonstrated to contribute to escape killing in neutrophil extracellular traps NETs. To further determine the effects of ssnA on virulence, the ssnA deletion mutant ΔssnA and its complemented strain C-ΔssnA were constructed. The ability of ΔssnA mutant to interact with human laryngeal epithelial cell Hep-2 was evaluated and it exhibited dramatically decreased ability to adhere to and invade Hep-2 cells. This mutation was found to exhibit significant attenuation of virulence when evaluated in CD1 mice, suggesting ssnA plays a critical role in the pathogenesis of SS2. Finally, we found that immunization with the ΔssnA mutant triggered both antibody responses and cell-mediated immunity, and conferred 80% protection against virulent SS2 challenge in mice. Taken together, our results suggest that ΔssnA represents an attractive candidate for designing an attenuated live vaccine against SS2.



Author: Miao Li, Ru-Jian Cai , Chun-Ling Li , Shuai Song, Yan Li, Zhi-Yong Jiang, Dong-Xia Yang

Source: http://plos.srce.hr/



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