Mycophenolate Mofetil Ameliorates Diabetic Nephropathy in db-db MiceReport as inadecuate




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BioMed Research International - Volume 2015 2015, Article ID 301627, 11 pages -

Research Article

Division of Nephrology, Department of Internal Medicine, College of Medicine, Kyung Hee University, Seoul 134-727, Republic of Korea

Division of Cardiology, Department of Internal Medicine, College of Medicine, Kyung Hee University, Seoul 134-727, Republic of Korea

Department of Pathology, College of Medicine, Kyung Hee University, Seoul 134-727, Republic of Korea

Received 4 February 2015; Revised 7 May 2015; Accepted 11 May 2015

Academic Editor: Joilson O. Martins

Copyright © 2015 Jung-Woo Seo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chronic low-grade inflammation is an important factor in the pathogenesis of diabetic complication. Mycophenolate mofetil MMF has an anti-inflammatory effect, inhibiting lymphocyte proliferation. Previous studies showed attenuation of diabetic nephropathy with MMF, but the underlying mechanisms were unclear. This study aimed to identify the effect of MMF on diabetic nephropathy and investigate its action mechanisms in type 2 diabetic mice model. Eight-week-old db-db and control mice db-m mice received vehicle or MMF at a dose of 30 mg-kg-day for 12 weeks. MMF-treated diabetic mice showed decreased albuminuria, attenuated mesangial expansion, and profibrotic mRNA expressions despite the high glucose level. The number of infiltrated CD4

and CD8

T cells in the kidney was significantly decreased in MMF-treated db-db mice and it resulted in attenuating elevated intrarenal TNF-α and IL-17. The renal chemokines expression and macrophages infiltration were also attenuated by MMF treatment. The decreased expression of glomerular nephrin and WT1 was recovered with MMF treatment. MMF prevented the progression of diabetic nephropathy in db-db mice independent of glycemic control. These results suggest that the effects of MMF in diabetic nephropathy are mediated by CD4

T cell regulation and related cytokines.





Author: Jung-Woo Seo, Yang Gyun Kim, Sang Ho Lee, Arah Lee, Dong-Jin Kim, Kyung-Hwan Jeong, Kyung Hye Lee, Seung Joon Hwang, Jong

Source: https://www.hindawi.com/



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