Antioxidative Activities and Active Compounds of Extracts from Catalpa Plant LeavesReport as inadecuate




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The Scientific World Journal - Volume 2014 2014, Article ID 857982, 7 pages -

Research Article

College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China

College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China

Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research YIC, Chinese Academy of Sciences CAS, Yantai 264003, China

Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received 14 July 2014; Revised 28 August 2014; Accepted 13 October 2014; Published 5 November 2014

Academic Editor: Marcela A. Segundo

Copyright © 2014 Hongyu Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In order to screen the Catalpa plant with high antioxidant activity and confirm the corresponding active fractions from Catalpa ovata G. Don, C. fargesii Bur., and C. bungei C. A. Mey., total flavonoid contents and antioxidant activities of the extracts-fractions of Catalpa plant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content in C. bungei C. A. Mey. 30.07 mg-g·DW was the highest, followed by those in C. fargesii Bur. 25.55 mg-g·DW and C. ovata G. Don 24.96 mg-g·DW. According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves of C. bungei C. A. Mey., the total flavonoid content and antioxidant activity in crude extracts from C. bungei C. A. Mey. 6 CA6 leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate EA fraction in ethanol crude extracts in CA6 leaves were the highest, followed by n-butanol, petroleum ether PE, and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin 1 and apigenin 2, respectively.





Author: Hongyu Xu, Gege Hu, Juane Dong, Qin Wei, Hongbo Shao, and Ming Lei

Source: https://www.hindawi.com/



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