Extended-Spectrum beta β-Lactamases and Antibiogram in Enterobacteriaceae from Clinical and Drinking Water Sources from Bahir Dar City, EthiopiaReport as inadecuate




Extended-Spectrum beta β-Lactamases and Antibiogram in Enterobacteriaceae from Clinical and Drinking Water Sources from Bahir Dar City, Ethiopia - Download this document for free, or read online. Document in PDF available to download.

Background

The spread of Extended-Spectrum beta β-Lactamases ESBL-producing Enterobacteriaceae has become a serious global problem. ESBL-producing Enterobacteriaceae vary based on differences in antibiotic use, nature of patients and hospital settings. This study was aimed at determining ESBL and antibiogram in Enterobacteriaceae isolates from clinical and drinking water sources in Bahir Dar City, Northwest Ethiopia.

Methods

Enterobacteriaceae species were isolated from clinical materials and tap water using standard culturing procedures from September 2013 to March 2015. ESBL-producing-Enterobacteriaceae were detected using double-disk method by E-test Cefotaxim-cefotaxim+ clavulanic acid and Ceftazidime-ceftazidime+ clavulanic acid BioMerieux SA, France on Mueller Hinton agar Oxoid, UK.

Results

Overall, 274 Enterobacteriaceae were isolated. Of these, 210 44% were from patients and 64 17.1% were from drinking water. The median age of the patients was 28 years. Urinary tract infection and blood stream infection accounted for 60% and 21.9% of Enterobacteriaceae isolates, respectively. Klebsiella pneumoniae was isolated from 9 75% of neonatal sepsis. The overall prevalence of ESBL-producing Enterobacteriaceae in clinical and drinking water samples were 57.6% and 9.4%, respectively. The predominant ESBL-producers were K. pneumoniae 34 69.4% and Escherichia coli 71 58.2%. Statistically significant associations were noted between ESBL-producing and non- producing Enterobacteriaceae with regard to age of patients, infected body sites and patient settings P = 0.001. ESBL-producing Enterobacteriaceae showed higher levels of resistance against chloramphenicol, ciprofloxacin and cotrimoxazole than non-ESBL producers P = 0.001

Conclusions

ESBL-producing Enterobacteriaceae coupled with high levels of other antimicrobials become a major concern for treatment of patients with invasive infections such as blood stream infections, neonatal sepsis and urinary tract infections. ESBL-producing Enterobacteriaceae were also detected in drinking water sources.



Author: Bayeh Abera , Mulugeta Kibret, Wondemagegn Mulu

Source: http://plos.srce.hr/



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