Molecular Characterization of Resistance to Soybean Rust Phakopsora pachyrhizi Syd. and Syd. in Soybean Cultivar DT 2000 PI 635999Report as inadecuate




Molecular Characterization of Resistance to Soybean Rust Phakopsora pachyrhizi Syd. and Syd. in Soybean Cultivar DT 2000 PI 635999 - Download this document for free, or read online. Document in PDF available to download.

Resistance to soybean rust SBR, caused by Phakopsora pachyrhizi Syd. and Syd., has been identified in many soybean germplasm accessions and is conferred by either dominant or recessive genes that have been mapped to six independent loci Rpp1 –Rpp6, but No U.S. cultivars are resistant to SBR. The cultivar DT 2000 PI 635999 has resistance to P. pachyrhizi isolates and field populations from the United States as well as Vietnam. A F6:7 recombinant inbred line RIL population derived from Williams 82 × DT 2000 was used to identify genomic regions associated with resistance to SBR in the field in Ha Noi, Vietnam, and in Quincy, Florida, in 2008. Bulked segregant analysis BSA was conducted using the soybean single nucleotide polymorphism SNP USLP 1.0 panel along with simple sequence repeat SSR markers to detect regions of the genome associated with resistance. BSA identified four BARC SNP markers near the Rpp3 locus on chromosome Chr. 6. Genetic analysis identified an additional genomic region around the Rpp4 locus on Chr. 18 that was significantly associated with variation in the area under disease progress curve AUDPC values and sporulation in Vietnam. Molecular markers tightly linked to the DT 2000 resistance alleles on Chrs. 6 and 18 will be useful for marker-assisted selection and backcrossing in order to pyramid these genes with other available SBR resistance genes to develop new varieties with enhanced and durable resistance to SBR.



Author: Tri D. Vuong , David R. Walker, Binh T. Nguyen, Tuyet T. Nguyen, Hoan X. Dinh, David L. Hyten, Perry B. Cregan, David A. Sleper,

Source: http://plos.srce.hr/



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