Brazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-Forming Endothelial Cells through Inactivation of Survival Signal ERK1-2Report as inadecuate




Brazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-Forming Endothelial Cells through Inactivation of Survival Signal ERK1-2 - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative MedicineVolume 2011 2011, Article ID 870753, 8 pages

Original Article

Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka, Shizuoka 422-8526, Japan

Department of Food and Nutrition, Dong-A University, 840 Hadan-2 dong, Saha-gu, Busan 604-714, Republic of Korea

Department of Thoracic Oncology, Hyogo College of Medicine, Japan

Cancer Center, Hyogo College of Medicine, Hyogo 663-8501, Japan

Received 8 October 2008; Accepted 3 March 2009

Copyright © 2011 Kazuhiro Kunimasa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We recently reported that propolis suppresses tumor-induced angiogenesis through tube formation inhibition and apoptosis induction in endothelial cells. However, molecular mechanisms underlying such angiogenesis suppression by propolis have not been fully elucidated. The aim of this study was to investigate the effects of ethanol extract of Brazilian propolis EEBP on two major survival signals, extracellular signal-regulated kinase 1-2 ERK1-2 and Akt, and to elucidate whether changes in these signals were actually involved in antiangiogenic effects of the propolis. Detection by western blotting revealed that EEBP suppressed phosphorylation of ERK1-2, but not that of Akt. Pharmacological inhibition by U0126 demonstrated that ERK1-2 inactivation alone was enough to inhibit tube formation and induce apoptosis. It was also shown that EEBP and U0126 similarly induced activation of caspase-3 and cleavage of poly ADP-ribose polymerase PARP and lamin A-C, all of which are molecular markers of apoptosis. These results indicate that inhibition of survival signal ERK1-2, and subsequent induction of apoptosis, is a critical mechanism of angiogenesis suppression by EEBP.





Author: Kazuhiro Kunimasa, Mok-Ryeon Ahn, Tomomi Kobayashi, Ryoji Eguchi, Shigenori Kumazawa, Yoshihiro Fujimori, Takashi Nakano, T

Source: https://www.hindawi.com/



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